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High-specification 4-channel LED illumination system


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High-specification 4-channel LED illumination system for fluorescence. For use with a 3mm liquid light guide. Includes light source, control pod, power supply, & ZZ plug

The pE-4000 is a powerful 4-channel high-specification LED illumination system. It is flexible, controllable, and environmentally friendly.


  • Broad LED spectrum available: 365-770nm – match LED peaks to existing microscope filters
  • Four channel wavelength-grouping design matches with all commercially available dual, triple, and quad filters
  • Individual control of selected LED wavelengths – less background, no filter wheel or shutters
  • Integrated with many imaging software packages – can be incorporated into all experimental parameters
  • Instant on/off – no shutters required, no warm up, or cool down
  • Stable and repeatable – reliable and consistent results
  • Wide range of microscope adaptors – fits most microscopes
  • Precise intensity control in 1% steps (0 – 100%) – no neutral density (ND) filters required
  • Excellent uniformity over field of view – fixed and stable, no alignment necessary
  • Long lifetime – expected to exceed 25,000 hours of operating time


Ideal for multi-user and core facilities

  • Pre-sets allowing lab manager to match white spectrum to existing filter cubes
  • Higher contrast images from matched-white spectrum
  • Simple to fit, simple to use
  • No training required


Ideal for advanced research

  • Individual LED wavelength selection
  • Rapid switching between LED wavelengths enables capture of high speed events
  • Transistor–transistor logic (TTL) and USB interfaces with imaging packages
  • Excitation filters can be fitted in optical path for controlled switching with no moving parts
  • Analogue inputs for dynamic intensity control
  • Optical feedback for applications requiring higher stability
  • Internal function generator for electrophysiology and optogenetics applications


  • Very broad spectrum from 16 installed LEDs (365nm -770nm)
  • Choose 1 of 4 wavelengths for each 4 channels on the control pod
  • Unique wavelength grouping concept (see below)
  • Optimum wavelength to suit your experiment
  • Specific wavelength characterisation for optimal optogenetic control of cell responses

Wavelength grouping concept

CoolLED has developed a comprehensive understanding of the complexities of configuring and selecting filter sets for samples which have multiple stains. These multi-band filters must have a corresponding absorption and emission band for each stain and the bands must be kept spectrally separate to ensure there is no overlap.

DAPI/FITC/TRITC/Cy5 Quad filter excited by pE-4000 at matched wavelengths shown above.

This comes from recognising that all the stains used in multi-band work can have their absorption bands divided into four separate groups across the spectrum due to the restricted bandwidth available. This has allowed the development of a patent-pending, wavelength-grouping concept which makes it possible to deliver more power in an efficient four channel system.

With its unique 16 selectable LED wavelengths, the pE-4000 ensures optimum compatibility with all single and multi-band filter sets. The flexibility and extensive functionality of this product broadens the range of illumination options in core facilities.


  • Low power consumption – Huge savings compared to old lamp technology.
  • >25,000 hours operation
  • Highly stable, repeatable LED technology
  • High energy efficiency
  • Mercury Free

Cell viability

  • Optimal illumination through control features to extend fluorescence of cells
  • Reduced photobleaching and phototoxicity
  • Microsecond switching
  • Variable pulse duration on/off



  • Advanced integration into major microscope software platforms
  • Manual control pod
  • USB interface
  • High speed digital TTL triggering
  • Analogue control – dynamic intensity sinusoidal control option
  • White light control
  • Save wavelength combinations and return to previous set-up
  • Individual channel intensity control



  • High signal to noise ratio which gives cleaner images and data

Time series of a confluent sheet of keratinocytes migrating during a live-cell imaging outgrowth assay, acquired on an Olympus IX-83 microscope equipped with a CoolLED pE-4000 lightsource. Scale bar = 150 μm.


  • Fits to all major research and clinical microscopes
  • Delivers light through a liquid light guide or fibre
  • Offers inline removable excitation filter holders
  • Uses the Universal Collimator



  • 3 year warranty after registration
  • First year warranty swap

The compact pE-4000 light source houses all the LEDs, their combining optics, thermal management, and control electronics. A dual mode control pod gives manual control in either white or advanced modes. Light is delivered to the microscope by either a single liquid light guide (LLG) or fibre. LLGs can be used in conjunction with CoolLED’s pE-Universal Collimator which can accept a microscope adaptor from CoolLED’s extensive range.


To view the accessories available for this product please see the PDF file in the downloads tab.

pE-4000 Datasheet
pE-4000 Manual
pE-4000 Accessories

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