High-specification 4-channel LED illumination system for fluorescence. For use with a 3mm liquid light guide. Includes light source, control pod, power supply, & ZZ plug
The pE-4000 is a powerful 4-channel high-specification LED illumination system. It is flexible, controllable, and environmentally friendly.
Features
- Broad LED spectrum available: 365-770nm – match LED peaks to existing microscope filters
- Four channel wavelength-grouping design matches with all commercially available dual, triple, and quad filters
- Individual control of selected LED wavelengths – less background, no filter wheel or shutters
- Integrated with many imaging software packages – can be incorporated into all experimental parameters
- Instant on/off – no shutters required, no warm up, or cool down
- Stable and repeatable – reliable and consistent results
- Wide range of microscope adaptors – fits most microscopes
- Precise intensity control in 1% steps (0 – 100%) – no neutral density (ND) filters required
- Excellent uniformity over field of view – fixed and stable, no alignment necessary
- Long lifetime – expected to exceed 25,000 hours of operating time
Ideal for multi-user and core facilities
- Pre-sets allowing lab manager to match white spectrum to existing filter cubes
- Higher contrast images from matched-white spectrum
- Simple to fit, simple to use
- No training required
Ideal for advanced research
- Individual LED wavelength selection
- Rapid switching between LED wavelengths enables capture of high speed events
- Transistor–transistor logic (TTL) and USB interfaces with imaging packages
- Excitation filters can be fitted in optical path for controlled switching with no moving parts
- Analogue inputs for dynamic intensity control
- Optical feedback for applications requiring higher stability
- Internal function generator for electrophysiology and optogenetics applications
Selectability
- Very broad spectrum from 16 installed LEDs (365nm -770nm)
- Choose 1 of 4 wavelengths for each 4 channels on the control pod
- Unique wavelength grouping concept (see below)
- Optimum wavelength to suit your experiment
- Specific wavelength characterisation for optimal optogenetic control of cell responses
Wavelength grouping concept
CoolLED has developed a comprehensive understanding of the complexities of configuring and selecting filter sets for samples which have multiple stains. These multi-band filters must have a corresponding absorption and emission band for each stain and the bands must be kept spectrally separate to ensure there is no overlap.
DAPI/FITC/TRITC/Cy5 Quad filter excited by pE-4000 at matched wavelengths shown above.
This comes from recognising that all the stains used in multi-band work can have their absorption bands divided into four separate groups across the spectrum due to the restricted bandwidth available. This has allowed the development of a patent-pending, wavelength-grouping concept which makes it possible to deliver more power in an efficient four channel system.
With its unique 16 selectable LED wavelengths, the pE-4000 ensures optimum compatibility with all single and multi-band filter sets. The flexibility and extensive functionality of this product broadens the range of illumination options in core facilities.
Sustainability
- Low power consumption – Huge savings compared to old lamp technology.
- >25,000 hours operation
- Highly stable, repeatable LED technology
- High energy efficiency
- Mercury Free
Cell viability
- Optimal illumination through control features to extend fluorescence of cells
- Reduced photobleaching and phototoxicity
- Microsecond switching
- Variable pulse duration on/off
Controllability
- Advanced integration into major microscope software platforms
- Manual control pod
- USB interface
- High speed digital TTL triggering
- Analogue control – dynamic intensity sinusoidal control option
- White light control
- Save wavelength combinations and return to previous set-up
- Individual channel intensity control
Visibility
- High signal to noise ratio which gives cleaner images and data
Time series of a confluent sheet of keratinocytes migrating during a live-cell imaging outgrowth assay, acquired on an Olympus IX-83 microscope equipped with a CoolLED pE-4000 lightsource. Scale bar = 150 μm.
Versatile
- Fits to all major research and clinical microscopes
- Delivers light through a liquid light guide or fibre
- Offers inline removable excitation filter holders
- Uses the Universal Collimator
Reliability
- 3 year warranty after registration
- First year warranty swap
The compact pE-4000 light source houses all the LEDs, their combining optics, thermal management, and control electronics. A dual mode control pod gives manual control in either white or advanced modes. Light is delivered to the microscope by either a single liquid light guide (LLG) or fibre. LLGs can be used in conjunction with CoolLED’s pE-Universal Collimator which can accept a microscope adaptor from CoolLED’s extensive range.
To view the accessories available for this product please see the PDF file in the downloads tab.