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Simple white light LED illumination system for fluorescence microscopy


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pE-300lite simple white light LED illumination system – single band. Direct fit SB filter configuration. Includes light source, control pod, power supply, YYY adaptor, and ZZ Plug

The pE-300lite is a white light LED illumination unit. It provides a broad spectrum of illumination, covering the excitation bands of common fluorescent stains. Operation is by a remote manual control pod with instant on/off and intensity control from 0 – 100%. It allows adjustment of output in 1% steps, giving precise control. The broad spectrum covers everyday fluorophores: DAPI, cyan fluorescent protein (CFP), Aqua, fluorescein isothiocyanate (FITC), tetramethylrhodamine (TRITC), texas red, Cy5 and many more.

It is now possible to view and image your samples brighter than before.

Convaleria taken with Photometrics Prime camera with 10 ms exposure.

LED illumination means no mercury to dispose of and lower energy costs due to low power consumption and the precise control offered. The pE-300lite can use all 3 channels at full power consuming under 60 Watts. This system comprises a pE-300lite light source, control pod, and power supply. It can be specified with a microscope adaptor for direct fit configuration or with a 3 mm diameter liquid light guide if there is a need to keep the source of illumination remote from the microscope. An optional pE-Universal Collimator and microscope adaptor can be selected for use with a liquid light guide.

As a replacement to a metal halide source on a confocal microscope, the liquid light guide configuration is particularly suitable for pre-aligning samples before scanning commences. In this way, photo bleaching of the sample can be minimised when LED illumination is used.

With an expected lifetime of over 25,000 hours and a comprehensive range of microscope adaptors, the pE-300lite white light LED illumination system can be fitted to most current and older microscopes and operate for many years without aligning or replacing bulbs. The result is a safe, convenient illumination system without any additional operating costs.

There are two pE-300lite configuration options:

  • Direct-fit for connecting to microscopes – by selecting from a range of microscope adaptors which covers all current and most older models. A simple once-only adjustment will allow optimisation to the optical path of the microscope.
  • Liquid light guide with a fixed 3mm diameter. An optional pE-Universal Collimator can be specified in conjunction with a microscope adaptor if required. CoolLED now includes a light source stand for the pE-300lite illumination systems ordered with a liquid light guide (see picture 3 on the product pictures).


CoolLED’s pE-300 Series was the winner of the ‘Go Beyond Award’ in the ‘Products’ category for ‘Excellence in sustainability in laboratory and other high technology facilities’. The International Institute for Sustainable Laboratories (I2SL) runs a unique awards program honouring organisations, individuals, products, and projects that are advancing sustainable, high-performance facilities.


The pE-300 Series is now ACT Label certified

With almost 40% of energy at university campuses being used by laboratories, steps need to be taken to increase the sustainability of lab technology. That’s why CoolLED strived to achieve ACT Label accreditation for the pE-300 Series.


What is ACT?

Introduced by My Green Lab, the ACT Label stands for accountability, consistency, and transparency. It’s a means of measuring the environmental impact factor of a product by reviewing the environmental impact of the product itself, its manufacturing process, packaging, and disposal.


Simple to buy

  • Work with everyday fluorophores/stains
  • Affordable through lab consumables budget
  • Broad spectrum – covers UV (DAPI) to Red region (Cy5)
  • Specify for existing single and multi-band filter sets – no need to buy new filters


Simple to fit

  • Direct fit to the microscope in seconds
  • Wide range of microscope adaptors – fits most microscopes
  • No alignment, a once only adjustment

Simple to use

  • Instant on/off – No shutters required, no warm up or cool down
  • Stable & repeatable – reliable and consistent results
  • Precise intensity control in 1% steps (0 – 100%) – no neutral density (ND) filters required
  • Excellent uniformity over field of view – fixed and stable, no alignment necessary
  • Long lifetime – expected to exceed 25,000 hours of operating time
  • No bulb replacement or ongoing consumables
pE-300 Datasheet
pE-300 Manual

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