Laser 2000

Super-resolution & Confocal Modules

Affordable and excellent add-on for imaging facilities to increase the imaging capacity.
Easily capture and analyse 3D images of cellular structures down to the single molecule level.
Re-scan Confocal Microscope

Super-resolution confocal microscopy can be used to achieve image resolution higher than that imposed by the diffraction limit.

The RCM rescan confocal microscope is available for both visible wavelengths and NIR wavelengths and offers scanning speeds of up to 4 frames per second. Lateral resolution for the visible is 170 nm and 240 nm for the NIR.

The SPINDLE® family of 3D microscope upgrades extend the capabilities of your microscope for nano-scale 3D imaging and sensing.

The SPINDLE attaches between any widefield microscope and camera and ensures pupil plane alignment to your microscope and objectives with corrective optics. It is available as a single or dual channel with up to four wavelengths with multicolour masks. The bypass mode allows you to revert back to 2D imaging without having to dismantle. It unlocks vast potential when used with other tools and techniques, including STORM, PALM, SOFI, widefield, TIRF, FRET, confocal, and more.

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5 channels, Visible plus Near-Infrared (NIR) Imaging RCM2.5 is the next big thing in confocal microscopy. Building on the existing RCM2 platform, we have developed a version that can utilise up to 5 lasers; extending over the Visible and Near-Infrared (NIR). Moreover, RCM2.5 enables you to use the latest advances in NIR dye development and […]
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We offer the new NL5 fast confocal microscope, ideal if you have any spinning disks which are nearing end of life as it can be a direct replacement for them. It is 16x brighter than spinning disk and due to the slit pinhole does not lead to pinhole crosstalk which effects a spinning disk system. This means that you can image much deeper and makes it very suitable for organoids, 3D live cells and embryos
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RCM2 is our second generation RCM, with digital scanner technology. It makes bi-directional scanning the standard and allows a speed of 2fps at 512×512 pixels. RCM2 has optics to make it suitable for super-resolution imaging with high NA objectives in the low magnification range, like 40x 1.4. A lower magnification allows for a bigger field of view (FOV), brighter images, and even lower laser power. RCM2 has demonstrated imaging at 10 nano-watt excitation power!
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Re-scan Confocal Microscope
RCM1 is a highly sensitive super-resolution confocal system based on the Re-scan Confocal Microscope (RCM) principle. Capture datasets with 170nm raw resolution (120nm after deconvolution) by using 60x to 100x high numerical aperture (NA) magnification objectives and keep the laser intensity at a minimum. RCM1 acquires super-resolution images with only 85 nano-watts laser power
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SPINDLE 3D Microscope Upgrade
Double Helix Optics’ SPINDLE™ gives researchers the ability to easily capture and analyse 3D images of cellular structures down to the single molecule level. Built-in bypass mode allows for easy return to non-3D experiments
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The RCM Rescan Confocal Microscope was created by Confocal.nl

Confocal.nl was founded in 2016 by Dr Erik Manders and Peter Drent. Erik is the head of the research group that developed the RCM technology and continues to work on the future models. He is well known in the microscopy world by the Manders’ coefficient and the Controlled Light Exposure Microscopy (CLEM, together with Ron Hoebe). Peter has played a key role in the development of Confocal.nl and has more than 25 years of experience in the microscopy business.

The company currently focusses on combining super-resolution capability with higher sensitivity than most confocal microscopes.


 

Double Helix is a team of top talent from a range of fields coming together to advance discovery and research in precision 3D nano-imaging. They have recognised world experts in super-resolution, first-rate engineers in optics, 3D imaging, software, and advisors from the research and business world. They are experts in their fields, collaborators in others, and excited by discovery.

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