Laser 2000

Filter Sets by Fluorophore

Find Semrock filter sets compatible with many popular fluorophores.

Many popular fluorophores are shown in the table to the right.

Select your chosen fluorophore and click on its name to find compatible filter sets.

Model and evaluate the spectral performance of fluorophores, filter sets, light sources, and detectors as components of an overall system with Semrock’s SearchLight, a free, online spectral plotting and analysis tool.

Note: Semrock does not produce or sell fluorophores. Actual peak wavelengths may vary for different applications and are to be used for guidance purposes only.

Fluorophore Peak Absorption Wavelength (nm) Peak Emission Wavelength (nm)
1,8-ANS 372 477
2-dodecylresorufin-lipid 582 595
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-carboxynapthofluorescein (pH 10) 555 615
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
6-carboxyrhodamine 6G 525 555
6-JOE 520 548
7-AAD (7-aminoactinomycin D) 548 648
7-amino-4-methylcoumarin (AMC) 346 442
7-hydroxy-4-methylcoumarin 360 442
ACMA (9-amino-6-chloro-2-methoxyacridine) 419 483
Acridine Orange (+DNA) 502 526
Acridine Orange (+RNA) 460 650
Acridine Yellow 470 550
Alexa Fluor® 350 343 441
Alexa Fluor® 405 401 422
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 514 518 543
Alexa Fluor® 532 534 553
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
Alexa Fluor® 633 631 647
Alexa Fluor® 635 633 648
Alexa Fluor® 647 653 668
Alexa Fluor® 660 663 691
Alexa Fluor® 680 679 702
Alexa Fluor® 700 696 719
Alexa Fluor® 750 751 776
AMCA (Aminomethylcoumarin) 350 448
AmCyan1 458 489
Amplex UltraRed peroxidation product (pH 7.5) 568 581
APC 651 660
APC (Allophycocyanin) 651 660
Aqua 431 430 478
AsRed 2 576 592
ATTO 390 392 477
ATTO 425 436 484
ATTO 465 453 507
ATTO 488 500 525
ATTO 495 495 527
ATTO 520 516 538
ATTO 532 501 545
ATTO 550 553 576
ATTO 565 563 592
ATTO 590 594 624
ATTO 594 601 624
ATTO 633 629 658
ATTO 635 635 658
ATTO 647 644 670
ATTO 647N 645 668
ATTO 655 663 683
ATTO 680 680 700
ATTO 700 699 719
ATTO 725 727 754
ATTO 740 740 764
AzamiGreen 494 505
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BD Horizon V450 406 449
BD Horizon V500 410 500
BFP (EBFP) 380 440
BOBO™-1 461 484
BOBO™-3 570 605
BODIPY 630/650-X 625 641
BODIPY 650/665-X 646 664
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
BODIPY R6G 528 547
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
BODIPY TR-X phallacidin 590 621
BV421 – BD Horizon Brilliant™ Violet 421 407 421
BV480 – BD Horizon Brilliant™ Violet 480 440 479
Calcein 494 514
Calcium Crimson 589 609
Calcium Green-1 507 529
Calcium Orange 589 609
Calcofluor White 351 444
Carboxynaphthofluorescein 599 674
Cascade Blue™ 401 419
Cascade Yellow™ 399 549
CellTrace BODIPY TR methyl ester 598 625
CellTrace calcein violet 400 452
CellTracker Red CMTPX 586 614
CellTracker Violet BMQC+GSH 406 526
Cerulean 434 473
CFP (ECFP) 433 475
CFP2 490 510
ChlorophyllA 480 650
Cy2™ 492 507
Cy3.5™ 578 591
Cy3™ 554 566
Cy5.5™ 672 690
Cy5™ 649 666
Cy7™ 753 775
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DAPI 359 461
Dapoxyl 373 579
DDAO 648 657
DEAC 432 472
Dendra2 (Green) 491 507
Dendra2 (Red) 554 569
Di-8-ANEPPS 467 631
DiA 457 586
DiD (DiIC18(5)) – Lipophilic Tracer 648 670
DiFMU-pH 9.0 358 450
DiI 551 569
DiO 475 500
DiR 750 782
Dronpa 502 516
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-415 418 470
DY-505-Phalloidin 503 530
DY-530 537 560
DY-590 582 599
DY-630 634 656
DY-635 646 670
DY-675 675 699
DY-781 784 802
DyLight 350 351 434
DyLight 405 398 420
DyLight 549 553 569
DyLight 594 593 618
DyLight 633 620 649
DyLight 649 651 673
DyLight 680 676 705
DyLight 750 751 778
DyLight 800 777 794
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
Eosin 525 546
ER-Tracker™ Blue-White DPX 372 557
Ethidium bromide 518 603
Ethidium homodimer 527 617
evoglow-Bs1 449 496
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
Fluoro-Gold™ 365 418
FluoSpheres Blue fluorescent microspheres 356 414
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Dark Red fluorescent microspheres 656 683
FluoSpheres Orange fluorescent microspheres 540 559
FluoSpheres Red fluorescent microspheres 577 607
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
FM 4-64 505 725
Fura Red™ (high pH) 572 657
Fura-2 (calcium bound) 336 505
Fura-2 (calcium free) 366 515
GFP (EGFP) 489 511
GFPuv 395 509
Gold 525 521 551
Gold 550 551 564
Green 496 496 520
Green 500 501 524
HcRed1 588 618
HCS CellMask Red cytoplasmic 622 644
HCS LipidTOX Deep Red neutral lipid stain 635 652
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 405 403 428
HiLyte Fluor™ 488 497 526
HiLyte Fluor™ 555 550 566
HiLyte Fluor™ 594 592 616
HiLyte Fluor™ 647 649 673
HiLyte Fluor™ 680 688 702
HiLyte Fluor™ 750 750 778
Hoechst 33258 352 455
Hoechst 33342 352 455
Hoechst 34580 392 440
ICG 768 807
IRDye 680 NHS Ester 683 710
IRDye 700Phosphoramidite 691 699
IRDye 800 CW-MeOH 778 801
IRDye 800CW 775 792
IRDye 800CW-NHS Ester 775 792
IRDye 800RS 787 812
JC-1 499 525
JO-JO-1, JO-PRO-1 530 544
KFP-Red 575 599
LDS 751 560 711
LIVE-DEAD Fixable Aqua Dead Cell Stain 367 526
LIVE-DEAD Fixable Blue Dead Cell Stain 343 441
LIVE-DEAD Fixable Far Red Dead Cell Stain 653 668
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LIVE-DEAD Fixable Near-IR Dead Cell Stain 752 776
LIVE-DEAD Fixable Red Dead Cell Stain 595 613
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LOLO-1 568 580
Lucifer yellow 428 544
LysoSensor Blue 374 424
LysoSensor Green 448 502
LysoTracker Blue 373 422
LysoTracker Green 501 509
LysoTracker Red 573 592
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
Magnesium Orange 550 574
mApple 566 594
Marina Blue 363 461
mBBr+GSH 394 490
mCherry 587 610
Merocyanine 540 559 579
mHoneyDew 478 562
MitoTracker™ Deep Red 641 662
MitoTracker™ Green 490 512
MitoTracker™ Orange 551 575
MitoTracker™ Red 578 598
mKate2 588 633
mKO 549 559
mOrange 546 562
mOrange2 551 565
mPlum 589 649
mRFP 555 583
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
mTFP1 462 492
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Nile blue-EtOH 631 660
Nile Red 551 579
Nile red-phospolipid 553 637
Nile red-triglyceride 510 583
OFP 547 567
Orange 552 552 574
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
Pacific Blue™ 404 455
Pacific Orange 400 551
PerCP 490 677
pHrodo™, succinimidyl ester 560 587
Phycoerythrin (R-Phycoerythrin) 565 575
PKH67 488 500
POPO-1 433 457
POPO-3 533 574
PO-PRO-1 433 456
PO-PRO-3 539 570
Propidium Iodide (PI) 305 617
Pro-Q Diamond 556 583
Pro-Q Emerald 289 530
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
Qdot® 565 Nanocrystals 300 564
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
Qdot® 655 Nanocrystals 300 654
Qdot® 705 Nanocrystals 300 702
Qdot® 800 Nanocrystals 300 792
Quasar® 705 688 706
Quinine sulfate 349 461
ratiometric pHluorin pH5 500 509
ReAsH-CCPGCC 592 606
Red 580 580 603
Red 650 649 665
Resorufin 571 584
Rhod-2 553 577
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodamine phalloidin 558 575
Rhodamine Red-X 573 591
Rhodol Green 497 524
R-phycoerythrin (PE) 565 576
sgBFP™ 387 451
sgGFP™ (super glow GFP) 472 506
Sirius 355 424
SNARF (carboxy) 514 Excitation pH6 549 587
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 6.0) 549 587
SNARF-1 514nm (ph 9.0) 576 638
Sodium Green 507 532
SpectrumAqua 434 481
SpectrumBlue 405 449
SpectrumFRed (Far Red) 650 673
SpectrumGold 530 556
SpectrumGreen 497 538
SpectrumOrange 554 587
SpectrumRed 587 615
Sulforhodamine 101-EtOH 578 593
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYPRO Ruby protein gel stain 469 631
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO 45 451 485
SYTO 59 622 644
SYTO 60 650 681
SYTO 61 618 650
SYTO 82 540 559
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Blue 444 470
SYTOX Green-DNA 504 524
SYTOX Orange 547 570
SYTOX Red-DNA 640 658
TagBFP 402 457
TagCFP 458 480
Tetramethylrhodamine dextran 554 582
Texas Red dextran 592 613
Texas Red DHPE 584 608
Texas Red® 592 614
Texas Red-X® 595 613
ThiolTracker Violet+GSH 404 526
TO-PRO-1 515 531
TO-PRO-3 642 657
TOTO-1 514 531
TOTO-3 642 661
TRITC (Tetramethylrhodamine) 552 578
Tryptophan 295 340
TurboFP635(Katushka) 591 638
TurboGFP 482 503
TurboRFP 553 573
TurboYFP 525 538
Venus 516 528
Vybrant DyeCycle Green 506 534
Vybrant DyeCycle Orange 519 563
Vybrant DyeCycle Violet 369 437
wtGFP (wild type GFP, non-UV excitation) 474 509
X-Rhod-1 Indicator 580 601
YFP (yellow GFP) 513 530
YO-PRO-1 491 506
YO-PRO-3 613 629
YOYO-1 491 508
YOYO-3 611 631
ZsYellow1 529 539
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