Laser 2000 offers the following accessories for use with the niji LED light source.
BNC Breakout Cable
BNC breakout cable for use with the niji LED light source for fluorescence microscopy, from Bluebox Optics.
Zoom Collimator for 3 mm light guide
This is a zoom collimator capable of light delivery using a microscope. Below you will be able to see an example of light delivery using an Olympus microscope (false coloured using ImageJ). The two images show g illumination at 405 nm (first image) and at 550 nm (second image). The contours detail the uniformity of the power distribution demonstrating the apochromacity and beam quality.
When adjusting the zoom collimator, the aim is to maximise the uniformity of the brightness of the illumination across a microscope slide.
Follow these 4 steps in order to gain the best practice for adjusting the illumination:
- Using a fluorescence reference slide, insert the reference slide into the microscope.
- Ensure that the niji LED light source is connected to the microscope, is powered up, and that the slide is illuminated.
- Adjust the rear lens (i.e. the lens nearest the light guide) until the slide is fully illuminated.
- Adjust the front lens (i.e. the lens furthest from the light guide) until the illumination is brought into focus.
We’ve designed and built an apochromatic zoom collimator with adaptor rings for the four main microscope brands:
- Leica
- Nikon
- Olympus
- Zeiss
If you have a requirement for a new design and build, we are easily able to provide this for you, on a 4-6 week lead time. If you would like more information on the currently available adaptors or would like a quotation on a custom adaptor please get in touch.