Discover fast and deep 3D live cell imaging with the NL5 Confocal Microscope System
With 25 fps scan speed, study fast cell dynamics and perform 4D imaging in optimised conditions. The use of low laser powers facilitates long term live-cell imaging such as mouse embryos, zebrafish, and organoids.
Our solutions are as clear as our imaging
Sharper. Larger field of view. Easy to use.
Sharp images, a large field of view and easy to use? It’s possible with Confocal.nl. Get more from your samples, with a lower phototoxicity and a higher sensitivity than conventional confocal microscopes and study live samples longer.
Our NL5 uses multiple laser lines and a camera as a detector. The low laser power required is live cell friendly: it prevents phototoxicity in your live samples and photobleaching of your fluorophores. Creating long-term time lapses for thick samples was never this accessible.
The benefits of NL5
Using NL5 with long working distance or silicon objectives, you can observe even deeper into your samples for a long time without losing any detail of the cell’s behaviour.
Obtain sharp images with a high signal-to-noise ratio even in samples with a low amount of epitopes or weak stainings. Get more from your samples.
Use even lower laser power to minimise phototoxicity and photobleaching during live-cell imaging.
Customise your NL5 confocal microscope system
- Choice of microscope bodies, e.g. Olympus IX83 – this body does an excellent job in combination with NL5. The 30 and 60x silicone immersion objectives give superior image quality and are especially good for live cell imaging.
- Hamamatsu Orca Flash 4 V3 – the high QE and low noise level of the Flash 4 V3 provide high resolution and exceptionally high SNR images in combination with NL5.
- 4 channel laser system – very stable and quiet laser engine with 405, 488, 561 and 640 laser lines.
- Tokai Hit stage top incubator – the compact Tokai Hit stage top incubator is easy to mount & remove. It is suitable for chamber slides, dishes and well plates. Happy cells guaranteed!
- LED illuminator – we like the LED light engines for their quietness and brightness. No more noisy machines and exchanging bulbs!
- Compatible with a variety of software – including micromanager, Volocity, Inscoper, and NIS Elements. PSF deconvolution is also available with Microvolution and SVI Huygens.