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BrightLine® long-pass laser filter set: 405 nm laser sources

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This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today’s state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from DAPI and other similar fluorophores while offering superb blocking of popular 405 nm or 375 nm solid-state laser diodes. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.

Looking for this filter set in one of the following cubes: NTE, OFF, OMF or ZHE?

Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted filter sets LF405/LP-C

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreACMA
Alexa Fluor 350
Alexa Fluor 405
BFP
DAPI
Hoechst 33258
Hoechst 33342
Hoechst 34580


LF405/LP-B-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

LF405/LP-B-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-390/40-25 Single Band Exciter 25 x 5.0 mm 1
Di02-R405-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1
BLP01-405R-25 Long Pass Emitter 25 x 3.5 mm 1

LF405/LP-B-L01

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’

LF405/LP-B-L01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-390/40-21.8-D Single Band Exciter 21.8 x 2.0 mm 1
Di02-R405-22×29 Single Band Dichroic 22.0 x 29.0 x 1.1 mm 1
BLP01-405R-23.3-D Long Pass Emitter 23.3 x 2.0 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
BLP01-405R_Spectrum
FF01-390_40_Spectrum
Di02-R405_Spectrum

* Time Limited Offer  |  Terms & Conditions Apply  |   Contact us for Further Details

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If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.