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BrightLine single-band filter set: Cy7 and similar fluorophores

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This filter set is ideal for use with Cy7™ and Alexa Fluor 750, as well as other similar near-infrared fluorophores. The longer excitation and emission wavelengths of these fluorophores enable deeper tissue penetration. The superb brightness and excellent signal-to-noise ratio of this filter set is also ideal since deep tissue imaging applications often also tend to yield very low light levels.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreAlexa Fluor 750
Cy7


Cy7-B-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard
  • Zero pixel shift: no

Cy7-B-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-708/75-25 Single Band Exciter 25 x 5.0 mm 1
FF02-809/81-25 Single Band Emitter 25 x 3.5 mm 1
FF757-Di01-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

Cy7-B-000-ZERO

  • Filter size: Standard
  • Zero pixel shift: yes

Cy7-B-000-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-708/75-25 Single Band Exciter 25 x 5.0 mm 1
FF02-809/81-25 Single Band Emitter 25 x 3.5 mm 1
FF757-Di01-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

Cy7-B-L01-ZERO

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’
  • Zero pixel shift: yes

Cy7-B-L01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-708/75-21.8-D Single Band Exciter 21.8 x 2.0 mm 1
FF02-809/81-23.3-D Single Band Emitter 23.3 x 2.0 mm 1
FF757-Di01-22×29 Single Band Dichroic 22.0 x 29.0 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-708_75_spectrum
FF02-809_81_Spectrum
FF757-Di01_Spectrum
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Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.