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BrightLine Basic single-band filter set: FITC and similar fluorophores

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This filter set is ideal for use with FITC and other popular green fluorophores. This hard-coated filter set is the best value on the market, delivering higher brightness than the comparable premium soft-coated filter sets with similar contrast. Want options? For hard-coated yet even more economical FITC filter sets optimized specifically for clinical applications, including a long-pass filter set, see our FITC-LP01-Clinical set. For the highest performance, try our flagship BrightLine FITC-3540C set. Compare BrightLine Basic filters to soft-coated filter sets and our highest-performance BrightLine filter sets.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
Blocking BrightLine filters have blocking far exceeding OD 5 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimised for microscopy applications using the SpecMaker™ fluorescence filter design.
General Specifications
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.
Physical Specifications (for standard parts – please contact us for custom sizes)
Size Standard size (fits most microscopes)
Leica ‘large’ set
Surface Quality (Scratch-Dig) 80-50
Exciter/Emitter Orientation Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation Reflective coating side should face toward light source and sample.
Fluorophores
Fluorophore 5-5-FAM
Alexa Fluor 488
BODIPY
FITC
Fluo-4
rsGFP

FITC-A-Basic-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

FITC-A-Basic-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-475/35-25 Single Band Exciter 25 x 5.0 mm 1
FF01-530/43-25 Single Band Emitter 25 x 3.5 mm 1
FF499-Di01-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

FITC-A-Basic-L01

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’

FITC-A-Basic-L01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-475/35-21.8-D Single Band Exciter 21.8 x 2.0 mm 1
FF01-530/43-23.3-D Single Band Emitter 23.3 x 2.0 mm 1
FF499-Di01-22×29 Single Band Dichroic 22.0 x 29.0 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-475_35_spectrum
FF01-530_43_spectrum
FF499-Di01_spectrum

* Time Limited Offer  |  Terms & Conditions Apply  |   Contact us for Further Details

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FITC-A-Basic

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