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BrightLine single-band filter set: DAPI and similar fluorophores

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Highest Contrast DAPI set on the market. The preferred DAPI set for most applications, this filter set is optimized for achieving exceptionally low noise (background) yet high brightness, resulting in unsurpassed contrast (signal-to-noise ratio). Images of typical cell samples with DAPI-stained nuclei exhibit more than twice the contrast of those made with the leading competitor’s set. The carefully optimized narrow, red-shifted exciter filter minimizes fluorophore photobleaching and sample phototoxicity. What’s more, unlike soft-coated filters and absorbing glass filters used for DAPI excitation, the unique hard-coated exciter does not ‘burn-out’ under the intense UV illumination of modern fluorescence light sources.

This set is the unparalleled choice for most situations, especially those involving digital imaging. For specialized applications where brightness trumps contrast, use our DAPI-5060C set, which has the highest brightness on the market.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.

Physical Specifications (for standard parts - please contact us for custom sizes)
Filter sizeOlympus 'Large' (FX)
SizeOlympus large-size (FX) set for U-M710I
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.

FluorophoreAlexa Fluor 350
Hoechst 33258
Hoechst 33342
Hoechst 34580
LysoSensor Blue
Marina Blue
Pacific Blue


  • Size: Olympus large-size (FX) set for U-M710I

DAPI-1160B-U02 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-387/11-32 Single Band Exciter 32 x 5.0 mm 1
FF02-447/60-32 Single Band Emitter 32 x 3.5 mm 1
FF409-Di03-32×44-FX Single Band Dichroic 32 x 44 x 1.1 mm 1


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Optical Density

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Semrock Spectral Plot Info

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Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.