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BrightLine® long-pass λ/2 flatness super-resolution laser filter set: 561 & 568 nm laser sources

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This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today’s state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from RFP (e.g., mCherry), Texas Red, and other similar fluorophores while offering superb blocking of popular 561 nm (solid-state) or 568 nm (Kr-ion gas) lasers. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.

Loose and cube mounted filter sets are certified to deliver dichroic flatness <= λ/2 PV per inch @ 632.8 nm.

Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?

Our previous version is still available for those cube configurations LF561/LP-C

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
Filter sizeStandard
SizeStandard size (fits most microscopes)
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreAlexa Fluor 568
Cy3
DsRed
HcRed1
mApple
mCherry
mRFP1
Rhodamine
Texas Red


LF561/LP-D-000

  • Size: Standard size (fits most microscopes)

LF561/LP-D-000 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF01-561/14-25Single Band Exciter25 x 5.0 mm1
BLP02-561R-25Long Pass Emitter25 x 3.5 mm1
Di03-R561-t1-25x36Single Band Dichroic25.2 x 35.6 x 1.1 mm1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-561_14_Spectrum
BLP02-561R_Spectrum
Di03-R561_Spectrum

* Time Limited Offer  |  Terms & Conditions Apply  |   Contact us for Further Details

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LF561/LP-D

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Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.