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BrightLine Basic single-band filter set: TRITC and similar fluorophores

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This hard-coated filter set is ideal for use with TRITC and similar fluorophores, and is the best value on the market, delivering high brightness and contrast.

Need the best signal and noise performance? Try Semrock’s highest-performance BrightLine TRITC-B filter set.

Note: Historical limitations in filter technology led to the widespread use of TRITC emission filters that are overly broad and red-shifted, causing the TRITC fluorescence to appear red. This BrightLine Basic TRITC-A-Basic set delivers that familiar ‘reddish’ appearance. In contrast, Semrock’s highest-performance TRITC-B set has an emission band peak in the orange region, and thus its fluorescence correctly appears orange, and not red.

 

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

 

 

Optical Damage Rating

Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.

Semrock Filter Family

BrightLine Basic™ Fluorescence Filters

Size

Leica 'large' set, Standard size (fits most microscopes)

Surface Quality (Scratch-Dig)

80-50

Dichroic Orientation

Reflective coating side should face toward light source and sample.

Exciter/Emitter Orientation

Arrow on ring indicates preferred direction of propagation of light.

Microscope Compatibility

BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.

Blocking

BrightLine filters have blocking far exceeding OD 5 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimised for microscopy applications using the SpecMaker™ fluorescence filter design

Fluorophore

Alexa Fluor 546, DiI, dTomato, Rhodamine, TRITC – "reddish" appearance

TRITC-A-Basic-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

TRITC-A-Basic-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-542/20-25 Single Band Exciter 25 x 5.0 mm 1
FF01-620/52-25 Single Band Emitter 25 x 3.5 mm 1
FF570-Di01-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

TRITC-A-Basic-L01

  • Size: Leica ‘large’ set
  • Filter size: Leica “Large”

TRITC-A-Basic-L01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-542/20-21.8-D Single Band Exciter 21.8 x 2.0 mm 1
FF01-620/52-23.3-D Single Band Emitter 23.3 x 2.0 mm 1
FF570-Di01-22×29 Single Band Dichroic 22.0 x 29.0 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-542_20_spectrum
FF01-620_52_Spectrum
FF570-Di01_spectrum
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TRITC-A-Basic

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