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BrightLine® Pinkel filter set: CFP, YFP, & mCherry and similar fluorophores, illuminated with LED-based light sources

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Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 5-filter ‘Pinkel’ triple-band set is designed for imaging of a sample that is simultaneously labeled with CFP, YFP, and mCherry, (or other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter and a triple-band emitter (these can be offered assembled in a microscope cube), plus three ‘no burn-out’ single-band exciters that are intended to be mounted in the LED based light source, or in an exciter filter wheel or slider.

Interested in learning how this filter set will perform with your light source? Try Semrock’s SearchLight plotting & calculation tool.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Sutter threaded set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreCerulean
CFP
mCherry
Texas Red
Venus
YFP


LED-CFP/YFP/mCherry-3X-A-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard
  • Zero pixel shift: no

LED-CFP/YFP/mCherry-3X-A-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF02-438/24-25 Single Band Exciter 25 x 5.0 mm 1
FF01-509/22-25 Single Band Exciter 25 x 5.0 mm 1
FF01-578/21-25 Single Band Exciter 25 x 5.0 mm 1
FF01-475/543/702-25 Triple Band Emitter 25 x 3.5 mm 1
FF459/526/596-Di01-25×36 Triple Band Dichroic 25.2 x 35.6 x 1.1 mm 1

LED-CFP/YFP/mCherry-3X-A-000-ZERO

  • Filter size: Standard
  • Zero pixel shift: yes

LED-CFP/YFP/mCherry-3X-A-000-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF02-438/24-25 Single Band Exciter 25 x 5.0 mm 1
FF01-509/22-25 Single Band Exciter 25 x 5.0 mm 1
FF01-578/21-25 Single Band Exciter 25 x 5.0 mm 1
FF01-475/543/702-25 Triple Band Emitter 25 x 3.5 mm 1
FF459/526/596-Di01-25×36 Triple Band Dichroic 25.2 x 35.6 x 1.1 mm 1

LED-CFP/YFP/mCherry-3X-A-L01

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’
  • Zero pixel shift: no

LED-CFP/YFP/mCherry-3X-A-L01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF02-438/24-25 Single Band Exciter 25 x 5.0 mm 1
FF01-509/22-25 Single Band Exciter 25 x 5.0 mm 1
FF01-578/21-25 Single Band Exciter 25 x 5.0 mm 1
FF01-475/543/702-23.3-D Triple Band Emitter 23.3 x 2.0 mm 1
FF459/526/596-Di01-22×29 Triple Band Dichroic 22.0 x 29.0 x 1.1 mm 1

LED-CFP/YFP/mCherry-3X-A-L01-ZERO

  • Filter size: Leica ‘Large’
  • Zero pixel shift: yes

LED-CFP/YFP/mCherry-3X-A-L01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF02-438/24-25 Single Band Exciter 25 x 5.0 mm 1
FF01-509/22-25 Single Band Exciter 25 x 5.0 mm 1
FF01-578/21-25 Single Band Exciter 25 x 5.0 mm 1
FF01-475/543/702-23.3-D Triple Band Emitter 23.3 x 2.0 mm 1
FF459/526/596-Di01-22×29 Triple Band Dichroic 22.0 x 29.0 x 1.1 mm 1

LED-CFP/YFP/mCherry-3X-A-S01

  • Size: Sutter threaded set
  • Filter size: Sutter Threaded
  • Zero pixel shift: no

LED-CFP/YFP/mCherry-3X-A-S01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF02-438/24-25-STR Single Band Exciter 25 mm 1
FF01-509/22-25-STR Single Band Exciter 25 mm 1
FF01-578/21-25-STR Single Band Exciter 25 mm 1
FF01-475/543/702-25 Triple Band Emitter 25 x 3.5 mm 1
FF459/526/596-Di01-25×36 Triple Band Dichroic 25.2 x 35.6 x 1.1 mm 1

LED-CFP/YFP/mCherry-3X-A-S01-ZERO

  • Filter size: Sutter Threaded
  • Zero pixel shift: yes

LED-CFP/YFP/mCherry-3X-A-S01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF02-438/24-25-STR Single Band Exciter 25 mm 1
FF01-509/22-25-STR Single Band Exciter 25 mm 1
FF01-578/21-25-STR Single Band Exciter 25 mm 1
FF01-475/543/702-25 Triple Band Emitter 25 x 3.5 mm 1
FF459/526/596-Di01-25×36 Triple Band Dichroic 25.2 x 35.6 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF02-438_24_spectrum
FF01-475_543_702_Spectrum
FF01-509_22_Spectrum
FF459_526_596_Di01_Spectrum
FF01-578_21_Spectrum
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LED-CFP/YFP/mCherry-3X-A

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