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BrightLine Basic long-pass filter set: FITC and similar fluorophores

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This long-pass filter set is optimized for all popular clinical tests based on blue excitation and green fluorescence. These include both direct (DFA) and indirect (IFA) immunofluorescent tests utilizing the FITC fluorochrome. Samples will exhibit amazing brightness and superior contrast for easy and accurate identification in test after test. Because it is a long-pass set, for visual applications it offers higher brightness and the ability to observe longer-wavelength (e.g. yellow, orange, and red) fluorescence from counter-stains such as Rhodamine and Evans Blue. For our best-value bandpass filter set, try FITC-A-Basic.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 5 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimised for microscopy applications using the SpecMaker™ fluorescence filter design.

General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.

Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Surface Quality (Scratch-Dig)80-50
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.

FluorophoreAcridine Orange


  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

FITC-LP01-Clinical-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FITC-Ex01-Clin-25 Single Band Exciter 25 x 5.0 mm 1
FITC-LP01-Clin-25 Long Pass Emitter 25 x 3.5 mm 1
FITC-Di01-Clin-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1


  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’

FITC-LP01-Clinical-L01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FITC-Ex01-Clin-21.8-D Single Band Exciter 21.8 x 2.0 mm 1
FITC-LP01-Clin-23.3-D Long Pass Emitter 23.3 x 2.0 mm 1
FITC-Di01-Clin-22×29 Single Band Dichroic 22.0 x 29.0 x 1.1 mm 1


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Optical Density

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Semrock Spectral Plot Info

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Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.