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BrightLine single-band multi-exciter filter set: FURA2 and similar fluorophores

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This four-filter BrightLine fluorescence set is specially designed to achieve high-performance fast ratiometric imaging of Ca2+ using Fura-2 as an indicator. The carefully optimized excitation filters provide superb balance of free and saturated Ca2+ signals, while minimizing the crosstalk and noise, resulting in the most accurate ratiometric measurements. The high signal throughput of the filter set permits reliable Fura-2 (Ca2+) measurements, even when using light sources of relatively limited ultraviolet output. Independent laboratories have measured this set to have 2-4X the free and saturated signals as compared with filters from the leading competitor.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Sutter threaded set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreFura-2
LysoSensor Yellow/Blue


FURA2-C-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard
  • Zero pixel shift: no

FURA2-C-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-340/26-25 Single Band Exciter 25 x 5.0 mm 1
FF01-387/11-25 Single Band Exciter 25 x 5.0 mm 1
FF01-510/84-25 Single Band Emitter 25 x 3.5 mm 1
FF409-Di03-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

FURA2-C-000-ZERO

  • Filter size: Standard
  • Zero pixel shift: yes

FURA2-C-000-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-340/26-25 Single Band Exciter 25 x 5.0 mm 1
FF01-387/11-25 Single Band Exciter 25 x 5.0 mm 1
FF01-510/84-25 Single Band Emitter 25 x 3.5 mm 1
FF409-Di03-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

FURA2-C-L01-ZERO

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’
  • Zero pixel shift: yes

FURA2-C-L01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-340/26-21.8-D Single Band Exciter 21.8 x 2.0 mm 1
FF01-387/11-21.8-D Single Band Exciter 21.8 x 2.0 mm 1
FF01-510/84-23.3-D Single Band Emitter 23.3 x 2.0 mm 1
FF409-Di03-22×29 Single Band Dichroic 22.0 x 29.0 x 1.1 mm 1

FURA2-C-S01

  • Size: Sutter threaded set
  • Filter size: Sutter Threaded
  • Zero pixel shift: no

FURA2-C-S01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-340/26-25-STR Single Band Exciter 25 mm 1
FF01-387/11-25-STR Single Band Exciter 25 mm 1
FF01-510/84-25 Single Band Emitter 25 x 3.5 mm 1
FF409-Di03-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

FURA2-C-S01-ZERO

  • Filter size: Sutter Threaded
  • Zero pixel shift: yes

FURA2-C-S01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-340/26-25-STR Single Band Exciter 25 mm 1
FF01-387/11-25-STR Single Band Exciter 25 mm 1
FF01-510/84-25 Single Band Emitter 25 x 3.5 mm 1
FF409-Di03-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-340_26_Spectrum
FF01-387_11_Spectrum
FF01-510_84_Spectrum
FF409-Di03_Spectrum
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FURA2-C

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If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.