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BrightLine Basic single-band filter set: Wild-type GFP and similar fluorophores

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This filter set is ideal for use with wild-type Green Fluorescent Protein (wtGFP), which is blue-shifted relative to the most common forms of GFP. This hard-coated filter set is the best value on the market, delivering higher brightness than the comparable premium soft-coated filter sets with better contrast.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 5 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimised for microscopy applications using the SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Surface Quality (Scratch-Dig)80-50
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreWtGFP


WGFP-A-Basic-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

WGFP-A-Basic-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-445/45-25 Single Band Exciter 25 x 5.0 mm 1
FF01-510/42-25 Single Band Emitter 25 x 3.5 mm 1
FF482-Di01-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

WGFP-A-Basic-L01

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’

WGFP-A-Basic-L01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-445/45-21.8-D Single Band Exciter 21.8 x 2.0 mm 1
FF01-510/42-23.3-D Single Band Emitter 23.3 x 2.0 mm 1
FF482-Di01-22×29 Single Band Dichroic 22.0 x 29.0 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-445_45_spectrum
FF01-510_42_spectrum
FF482-Di01_spectrum
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WGFP-A-Basic

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