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BrightLine® single-band laser filter set: 594 nm laser sources

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This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today’s state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from mCherry and other similar fluorophores while offering superb blocking of popular 593.5 nm (Double DPSS), 594 nm (HeNe), and 594.1 nm (DPSS) and 594+/-0.3 nm lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.

Looking for this filter set in one of the following cubes: NTE, OFF, OMF or ZHE?

Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted filter sets LF594-D

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreAlexa Fluor 594
mCherry
mKate2
Texas Red


LF594-C-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

LF594-C-000 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF01-591/6-25Single Band Exciter25 x 5.0 mm1
FF01-647/57-25Single Band Emitter25 x 3.5 mm1
Di02-R594-25x36Single Band Dichroic25.2 x 35.6 x 1.1 mm1

LF594-C-L01

  • Size: Leica 'large' set
  • Filter size: Leica 'Large'

LF594-C-L01 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF01-591/6-21.8-DSingle Band Exciter21.8 x 2.0 mm1
FF01-647/57-23.3-DSingle Band Emitter23.3 x 2.0 mm1
Di02-R594-22x29Single Band Dichroic22.0 x 29.0 x 1.1 mm1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-647_57_Spectrum
Di02-R594_Spectrum
FF01-591_6_Spectrum

* Time Limited Offer  |  Terms & Conditions Apply  |   Contact us for Further Details

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LF594-C

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If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.