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BrightLine® single-band filter set: mCherry and similar fluorophores

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This filter set is ideal for use with the monomeric fluorescent protein mCherry. It also works well for broader orange-to-red fluorophores like MitoTracker Red. It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Olympus large-size (FX) set for U-M710I
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreMApple
mCherry
mRFP1


mCherry-C-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard
  • Zero pixel shift: no

mCherry-C-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-562/40-25 Single Band Exciter 25 x 5.0 mm 1
FF02-641/75-25 Single Band Emitter 25 x 3.5 mm 1
FF593-Di03-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

mCherry-C-000-ZERO

  • Filter size: Standard
  • Zero pixel shift: yes

mCherry-C-000-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-562/40-25 Single Band Exciter 25 x 5.0 mm 1
FF02-641/75-25 Single Band Emitter 25 x 3.5 mm 1
FF593-Di03-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

mCherry-C-L01-ZERO

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’
  • Zero pixel shift: yes

mCherry-C-L01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-562/40-21.8-D Single Band Exciter 21.8 x 2.0 mm 1
FF02-641/75-23.3-D Single Band Emitter 23.3 x 2.0 mm 1
FF593-Di03-22×29 Single Band Dichroic 22.0 x 29.0 x 1.1 mm 1

mCherry-C-U02

  • Size: Olympus large-size (FX) set for U-M710I
  • Filter size: Olympus ‘Large’ (FX)
  • Zero pixel shift: no

mCherry-C-U02 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-562/40-32 Single Band Exciter 32 x 5.0 mm 1
FF02-641/75-32 Single Band Emitter 32 x 3.5 mm 1
FF593-Di03-32×44-FX Single Band Dichroic 32 x 44 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-562_40_spectrum
FF593-Di03_Spectrum
FF02-641_75_Spectrum

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