Laser 2000
Home > Products > Light Delivery & Control > Microscopy Filters > Filter Sets & Cubes > All Microscopy Filter Sets > BrightLine® single-band λ/2 flatness super-resolution laser filter set: 488 nm laser sources

BrightLine® single-band λ/2 flatness super-resolution laser filter set: 488 nm laser sources

Semrock Logo

This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today’s state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from GFP, FITC, and other similar fluorophores while offering superb blocking of popular 473 nm (solid-state) or 488 nm (Ar-ion gas and solid-state) lasers. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.

Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.

Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?

Our previous version is still available for those cube configurations LF488-C

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
Filter sizeStandard
SizeStandard size (fits most microscopes)
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreAlexa Fluor 488
BODIPY
Calcein
Cy2
FITC
GFP
MitoTracker Green
rsGFP
sgGFP
wtGFP


LF488-D-000

  • Size: Standard size (fits most microscopes)

LF488-D-000 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF02-482/18-25Single Band Exciter25 x 5.0 mm1
FF01-525/45-25Single Band Emitter25 x 3.5 mm1
Di03-R488-t1-25x36Single Band Dichroic25.2 x 35.6 x 1.1 mm1

Transmission

Loading chart data...

Optical Density

Loading chart data...

Semrock Spectral Plot Info
FF01-525_45_Spectrum
FF02-482_18_Spectrum
Di03-R488_Spectrum
BACK
TO TOP

Request a Demo

LF488-D

Ask the Expert

If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.