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BrightLine full-multiband laser filter set: 405, 488, 561, & 635 nm laser sources

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This full-multiband set is optimized for laser excitation over four bands – utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, a 559, 561.4 or 568.2 nm laser source for mCherry, and a 632.8, 635, or 647.1 nm laser source for Cy5. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.

Looking for this filter set in one of the following cubes: NTE, OFF, OMF or ZHE?

Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted filter sets LF405/488/561/635-B

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreCy5
DAPI
FITC
GFP
mCherry
mRFP1


LF405/488/561/635-A-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

LF405/488/561/635-A-000 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF01-390/482/563/640-25Quad Band Exciter25 x 5.0 mm1
FF01-446/523/600/677-25Quad Band Emitter25 x 3.5 mm1
Di01-R405/488/561/635-25x36Quad Band Dichroic25.2 x 35.6 x 1.1 mm1

LF405/488/561/635-A-L01

  • Size: Leica 'large' set
  • Filter size: Leica 'Large'

LF405/488/561/635-A-L01 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF01-390/482/563/640-21.8-DQuad Band Exciter21.8 x 2.0 mm1
FF01-446/523/600/677-23.3-DQuad Band Emitter23.3 x 2.0 mm1
Di01-R405/488/561/635-22x29Quad Band Dichroic22.0 x 29.0 x 1.1 mm1

Transmission

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Optical Density

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Semrock Spectral Plot Info
Di01-R405_488_561_635_Spectrum
FF01-390_482_563_640_Spectrum
FF01-446_523_600_677_Spectrum
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LF405/488/561/635-A

Ask the Expert

If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.