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BrightLine® Sedat filter set: DAPI, FITC, TRITC, & Cy5 and similar fluorophores, illuminated with LED-based light engines

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Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 9-filter ‘Sedat’ quad-band set is designed for high-speed, sequential imaging of DAPI, FITC, TRITC, and Cy5 (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a quad-band beamsplitter with four single-band emitters intended to be mounted in filter wheels and four single-band exciters which can be installed into the LED light engine or mounted in filter wheels.

Interested in learning how this filter set will perform with your light source? Try Semrock’s SearchLight spectral plotting & analysis tool.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Sutter threaded set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreCy5
DAPI
FITC
GFP
TRITC


LED-DA/FI/TR/Cy5-4X4M-B-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

LED-DA/FI/TR/Cy5-4X4M-B-000 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF01-378/52-25Single Band Exciter25 x 5.0 mm1
FF01-474/27-25Single Band Exciter25 x 5.0 mm1
FF01-554/23-25Single Band Exciter25 x 5.0 mm1
FF01-635/18-25Single Band Exciter25 x 5.0 mm1
FF01-432/36-25Single Band Emitter25 x 3.5 mm1
FF01-515/30-25Single Band Emitter25 x 3.5 mm1
FF01-595/31-25Single Band Emitter25 x 3.5 mm1
FF01-698/70-25Single Band Emitter25 x 3.5 mm1
FF409/493/573/652-Di02-25x36Quad Band Dichroic25.2 x 35.6 x 1.1 mm1

LED-DA/FI/TR/Cy5-4X4M-B-L01

  • Size: Leica 'large' set
  • Filter size: Leica 'Large'

LED-DA/FI/TR/Cy5-4X4M-B-L01 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF01-378/52-25Single Band Exciter25 x 5.0 mm1
FF01-474/27-25Single Band Exciter25 x 5.0 mm1
FF01-554/23-25Single Band Exciter25 x 5.0 mm1
FF01-635/18-25Single Band Exciter25 x 5.0 mm1
FF01-432/36-25Single Band Emitter25 x 3.5 mm1
FF01-515/30-25Single Band Emitter25 x 3.5 mm1
FF01-595/31-25Single Band Emitter25 x 3.5 mm1
FF01-698/70-25Single Band Emitter25 x 3.5 mm1
FF409/493/573/652-Di02-22x29Quad Band Dichroic22.0 x 29.0 x 1.1 mm1

LED-DA/FI/TR/Cy5-4X4M-B-S01

  • Size: Sutter threaded set
  • Filter size: Sutter Threaded

LED-DA/FI/TR/Cy5-4X4M-B-S01 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF01-378/52-25-STRSingle Band Exciter25 mm1
FF01-474/27-25-STRSingle Band Exciter25 mm1
FF01-554/23-25-STRSingle Band Exciter25 mm1
FF01-635/18-25-STRSingle Band Exciter25 mm1
FF01-432/36-25-STRSingle Band Emitter25 mm1
FF01-515/30-25-STRSingle Band Emitter25 mm1
FF01-595/31-25-STRSingle Band Emitter25 mm1
FF01-698/70-25-STRSingle Band Emitter25 mm1
FF409/493/573/652-Di02-25x36Quad Band Dichroic25.2 x 35.6 x 1.1 mm1

Transmission

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Optical Density

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