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Avant™ single-band filter set: Cy3™, Alexa Fluor 555 and similar fluorophores

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This filter set is designed for use with Cy3™ and Alexa Fluor 555 and works well with other orange fluorophores. The Avant™ filter set family delivers significant increases in fluorescence performance for small Stokes Shift fluorophores compared to standard filter sets. Steep spectral edges and high complementary OD blocking maximize signal while maintaining low bleedthrough and high signal to noise ratio. The new Precise Edge Placement capability ensures every filter set delivers consistent performance.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingAvant filters have blocking far exceeding OD 5 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityAvant filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
Filter sizeStandard
SizeStandard size (fits most microscopes)
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective surface marked with part number - Orient in direction of incoming light


Fluorophores
FluorophoreAlexa Fluor 555
Cy3
orange fluorophores


Avant-Cy3-000

  • Size: Standard size (fits most microscopes)

Avant-Cy3-000 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
AF01-535/40-25Single Band Exciter25 x 5.0 mm1
AF01-584/40-25Single Band Emitter25 x 3.5 mm1
AF560-Di01-25x36Single Band Dichroic25.2 x 35.6 x 1.1 mm1

Transmission

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Optical Density

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Semrock Spectral Plot Info
AF01_535_40_measured
AF01_584_40_measured
AF560_Di01_measured
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Avant-Cy3

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Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.