Laser 2000

Re-scan Confocal Microscope Upgrade

RCM2

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RCM2 is our second generation RCM, with digital scanner technology. It makes bi-directional scanning the standard and allows a speed of 2fps at 512×512 pixels. RCM2 has optics to make it suitable for super-resolution imaging with high NA objectives in the low magnification range, like 40x 1.4. A lower magnification allows for a bigger field of view (FOV), brighter images, and even lower laser power. RCM2 has demonstrated imaging at 10 nano-watt excitation power!

 

Improve your imaging experience with RCM2

  • Super-resolution (120nm) imaging with 40x 1.4NA objectives.
  • Larger FOV without increasing acquisition time.
  • Bidirectional scanner.
  • Excellent add-on to any widefield microscope.
  • Easy to use.

Hardware integration in third-party software.

 

Discover super-resolution imaging with RCM2

Capture datasets of 170nm resolution raw (120 after deconvolution) over a very large FOV. Study fast live-cell dynamics and perform 4D imaging in optimized conditions. The increased detection efficiency facilitates acquisition in low fluorescence conditions, like single-molecule detection (smFISH).

In combination with silicon objectives, RCM2 allows for deep 3D imaging of organoids, zebrafish embryos, or larger live samples.

The benefits of RCM2

Using RCM2 with a 40x 1.4 objective, you can see more cells at full resolution at once. A larger field of view increases the chances of getting the results you need.

Obtain sharp images with a high signal-to-noise ratio even in samples with a low amount of epitopes or weak stainings. Get more from your samples.

Use even lower laser power to minimize phototoxicity and photobleaching during live-cell imaging.

Getting super-resolution raw images, without averaging or integration, reduces the acquisition time and allows for a more precise analysis of the subcellular structures.

Working principle of the re-scan confocal microscope

Re-scan Confocal Microscopy (RCM) is a super-resolution technique based on a standard laser-scanning confocal system, extended with an optical re-scanning unit. The re-scanner writes the image directly onto a camera chip. By doubling the sweep of the re-scanning mirrors, the image is magnified on the camera chip without increasing the size of the re-scanning spot. This results in an increase in resolution up to 170 nm. The camera-based detection and open pinhole design offer a high signal-to-noise ratio, while maintaining confocal sectioning capability. The re-scanning principle is optics-mechanics only, achieving an improved resolution image without any post-processing.

To find out how the RCM can benefit your project, watch this detailed video (8:22 mins) on how it works:

 

Better signal-to-noise ratio

The unique open pinhole design and camera-based detection allow twice the QE (up to 95%) and a 4x improvement of the signal-to-noise ratio.

Improved lateral resolution

RCM offers up to 170 nm lateral resolution, 1.4x improvement over a regular confocal microscope. Further improvement possible with deconvolution, up to 120nm.

User-friendly operation

High-resolution images are achieved with one-click; there are no adjustable settings, and no post-processing is required.

Open system architecture

Easy integration with your preferred system components; microscope and camera connect via C-mount, laser via single mode fibre. Both commercial and open-source software drivers are available.

Affordability

Low-cost due to its unique camera-based design and flexible system architecture.

Detector

Camera

Resolution

170 nm raw image (120 nm after deconvolution)

Quantum Efficiency

up to 95%

Field of View

220 x 220 μm (40x, super-resolution)

Optimised for

100x, 60x, 40x (high NA)

Scan Speed

2fps @ 512×512 pixels

Model

Visible

Software

Micromanager, Volocity, NIS Elements, Zen, LAS X, Cellsens

PSF for deconvolution

Microvolution, SVI Huygens

Bypass mode

Yes

Confocal.nl RCM Brochure
Advantages of RCM vs PMT based Confocal Microscopes
White paper - Long term live cell imaging
Top Applications
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RCM2

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