RCM2 is our second generation RCM, with digital scanner technology. It makes bi-directional scanning the standard and allows a speed of 2fps at 512×512 pixels. RCM2 has optics to make it suitable for super-resolution imaging with high NA objectives in the low magnification range, like 40x 1.4. A lower magnification allows for a bigger field of view (FOV), brighter images, and even lower laser power. RCM2 has demonstrated imaging at 10 nano-watt excitation power!
Improve your imaging experience with RCM2
- Super-resolution (120nm) imaging with 40x 1.4NA objectives.
- Larger FOV without increasing acquisition time.
- Bidirectional scanner.
- Excellent add-on to any widefield microscope.
- Easy to use.
Hardware integration in third-party software.
Discover super-resolution imaging with RCM2
Capture datasets of 170nm resolution raw (120 after deconvolution) over a very large FOV. Study fast live-cell dynamics and perform 4D imaging in optimized conditions. The increased detection efficiency facilitates acquisition in low fluorescence conditions, like single-molecule detection (smFISH).
In combination with silicon objectives, RCM2 allows for deep 3D imaging of organoids, zebrafish embryos, or larger live samples.
The benefits of RCM2
Using RCM2 with a 40x 1.4 objective, you can see more cells at full resolution at once. A larger field of view increases the chances of getting the results you need.
Obtain sharp images with a high signal-to-noise ratio even in samples with a low amount of epitopes or weak stainings. Get more from your samples.
Use even lower laser power to minimize phototoxicity and photobleaching during live-cell imaging.
Getting super-resolution raw images, without averaging or integration, reduces the acquisition time and allows for a more precise analysis of the subcellular structures.
Working principle of the re-scan confocal microscope
Re-scan Confocal Microscopy (RCM) is a super-resolution technique based on a standard laser-scanning confocal system, extended with an optical re-scanning unit. The re-scanner writes the image directly onto a camera chip. By doubling the sweep of the re-scanning mirrors, the image is magnified on the camera chip without increasing the size of the re-scanning spot. This results in an increase in resolution up to 170 nm. The camera-based detection and open pinhole design offer a high signal-to-noise ratio, while maintaining confocal sectioning capability. The re-scanning principle is optics-mechanics only, achieving an improved resolution image without any post-processing.
To find out how the RCM can benefit your project, watch this detailed video (8:22 mins) on how it works:
Better signal-to-noise ratio
The unique open pinhole design and camera-based detection allow twice the QE (up to 95%) and a 4x improvement of the signal-to-noise ratio.
Improved lateral resolution
RCM offers up to 170 nm lateral resolution, 1.4x improvement over a regular confocal microscope. Further improvement possible with deconvolution, up to 120nm.
User-friendly operation
High-resolution images are achieved with one-click; there are no adjustable settings, and no post-processing is required.
Open system architecture
Easy integration with your preferred system components; microscope and camera connect via C-mount, laser via single mode fibre. Both commercial and open-source software drivers are available.
Affordability
Low-cost due to its unique camera-based design and flexible system architecture.