Laser 2000

521 nm Avant™ Dichroic Beamsplitter, 25 x 36 mm

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For optimal filter set performance, pair this dichroic with AF01-504/24 and AF01-539/27, which have been optimized for Yellow Fluorescent Protein (YFP), Venus, and other key fluorophores. Precise edge placement of the steep transition edge combined with very high reflection and transmission bands maximize fluorophore performance compared to standard dichroics.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
Transmission Band Tavg > 93% 525.5 – 580 nm
Reflection Band Ravg > 98% 470 – 516.5 nm
Transmission Wavelength 521 nm
FWHM Bandwidth >> 6λ P-V RWE @ 632.8 nm
General Specifications
Angle of Incidence 45 ± 1.5 °
Cone Half-angle 2 °
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.
Flatness / RWE Classification Standard Epi-fluorescence
Physical Specifications (for standard parts – please contact us for custom sizes)
Mounted / Unmounted Unmounted
Shape Rectangular
Size 25.2 x 35.6 x 1.1 mm
Transverse Dimensions, LxW 25.2 mm x 35.6 mm
Substrate Thickness Tolerance, Unmounted ± 0.05 mm
Filter Thickness, Mounted 1.05 mm
Filter Thickness Tolerance, Mounted ± 0.1 mm
Clear Aperture ≥ 80% (elliptical)
Surface Quality (Scratch-Dig) 60-40
Substrate Thickness 1.05 mm
Substrate Type Low-autofluorescence optical quality glass
Orientation Reflective surface marked with part number – Orient in direction of incoming light


  • Size: 25.2 x 35.6 x 1.1 mm


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Optical Density

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Semrock Spectral Plot Info

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