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BrightLine single-band filter set: Tryptophan and similar fluorophores

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Unique UV fluorescence filter set! Only Semrock offers true UV fluorescence filters with high transmission, edge steepness, and blocking that rival high-performance visible filters. The filters in this set are ideal for imaging the intrinsic fluorescence from aromatic amino acids (like tryptophan) and other UV-excited fluorescent species. Note: This set is optimized for systems using a light source (such as a UV LED or filtered Xe arc lamp) with no near-infrared (near-IR) intensity and/or a detector (such as a photomultiplier tube or IR-filtered CCD camera) that is not sensitive to near-IR light. It is not designed to block near-IR light – for applications involving a light source with appreciable near-IR intensity and a near-IR-sensitive detector, contact Semrock.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.

Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.



  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

TRP-A-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-280/20-25 Single Band Exciter 25 x 5.0 mm 1
FF01-357/44-25 Single Band Emitter 25 x 3.5 mm 1
FF310-Di01-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1


  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’

TRP-A-L01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-280/20-21.8-D Single Band Exciter 21.8 x 2.0 mm 1
FF01-357/44-23.3-D Single Band Emitter 23.3 x 2.0 mm 1
FF310-Di01-22×29 Single Band Dichroic 22.0 x 29.0 x 1.1 mm 1


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Optical Density

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Semrock Spectral Plot Info

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