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BrightLine single-band filter set: Spectrum Aqua and similar fluorophores

Clear

This filter set is optimised to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring Spectrum Aqua.

 

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

 

 

Optical Damage Rating

Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.

Semrock Filter Family

BrightLine® Fluorescence Filters

Size

Leica 'large' set, Olympus large-size (FX) set for U-M710I, Standard size (fits most microscopes)

Surface Quality (Scratch-Dig)

60-40

Dichroic Orientation

Reflective coating side should face toward light source and sample.

Exciter/Emitter Orientation

Arrow on ring indicates preferred direction of propagation of light.

Microscope Compatibility

BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.

Blocking

BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimised for microscopy applications using the SpecMaker™ fluorescence filter design

Fluorophore

CFP, DEAC, SpectrumAqua

SpAqua-C-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard
  • Zero pixel shift: no

SpAqua-C-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF02-438/24-25 Single Band Exciter 25 x 5.0 mm 1
FF01-483/32-25 Single Band Emitter 25 x 3.5 mm 1
FF458-Di02-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

SpAqua-C-000-ZERO

  • Filter size: Standard
  • Zero pixel shift: yes

SpAqua-C-000-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF02-438/24-25 Single Band Exciter 25 x 5.0 mm 1
FF01-483/32-25 Single Band Emitter 25 x 3.5 mm 1
FF458-Di02-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

SpAqua-C-L01-ZERO

  • Size: Leica ‘large’ set
  • Filter size: Leica “Large”
  • Zero pixel shift: yes

SpAqua-C-L01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF02-438/24-21.8-D Single Band Exciter 21.8 x 2.0 mm 1
FF01-483/32-23.3-D Single Band Emitter 23.3 x 2.0 mm 1
FF458-Di02-22×29 Single Band Dichroic 22.0 x 29.0 x 1.1 mm 1

SpAqua-C-U02

  • Size: Olympus large-size (FX) set for U-M710I
  • Filter size: Olympus “Large” (FX)
  • Zero pixel shift: no

SpAqua-C-U02 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF02-438/24-32 Single Band Exciter 32 x 5.0 mm 1
FF01-483/32-32 Single Band Emitter 32 x 3.5 mm 1
FF458-Di02-32×44-FX Single Band Dichroic 32 x 44 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-483_32_spectrum
FF02-438_24_spectrum
FF458-Di02_Spectrum
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SpAqua-C

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