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BrightLine® single-band filter set: mTFP (Teal) and similar fluorophores, illuminated with LED-based light sources

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This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring mTFP (Teal) (or other key fluorophores – see complete list above). This state-of-the-art set has been optimized for popular LED based light sources. This set has been designed to deliver significant fluorescence signal improvements for LED based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps.

Interested in learning how this filter set will perform with your light source?

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Olympus large-size (FX) set for U-M710I
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreAmCyan1
Aqua431
ATTO 425
BOBO-1
CFP
mTFP1 (Teal)
SpectrumAqua
TagCFP


LED-mTFP-A-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard
  • Zero pixel shift: no

LED-mTFP-A-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-442/42-25 Single Band Exciter 25 x 5.0 mm 1
FF01-494/34-25 Single Band Emitter 25 x 3.5 mm 1
FF470-Di01-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

LED-mTFP-A-000-ZERO

  • Filter size: Standard
  • Zero pixel shift: yes

LED-mTFP-A-000-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-442/42-25 Single Band Exciter 25 x 5.0 mm 1
FF01-494/34-25 Single Band Emitter 25 x 3.5 mm 1
FF470-Di01-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

LED-mTFP-A-L01-ZERO

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’
  • Zero pixel shift: yes

LED-mTFP-A-L01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-442/42-21.8-D Single Band Exciter 21.8 x 2.0 mm 1
FF01-494/34-23.3-D Single Band Emitter 23.3 x 2.0 mm 1
FF470-Di01-22×29 Single Band Dichroic 22.0 x 29.0 x 1.1 mm 1

LED-mTFP-A-U02

  • Size: Olympus large-size (FX) set for U-M710I
  • Filter size: Olympus ‘Large’ (FX)
  • Zero pixel shift: no

LED-mTFP-A-U02 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-442/42-32 Single Band Exciter 32 x 5.0 mm 1
FF01-494/34-32 Single Band Emitter 32 x 3.5 mm 1
FF470-Di01-32×44-FX Single Band Dichroic 32 x 44 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-442_42_Spectrum
FF01-494_34_Spectrum
FF470-Di01_Spectrum
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LED-mTFP-A

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If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.

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