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BrightLine single-band filter set: FITC and similar fluorophores

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This set is optimized for the most popular green fluorophores like FITC (fluorescein isothiocyanate), 5-FAM, and Alexa Fluor 488. It provides the highest signal-to-noise ratio (contrast) and is ideal for similar fluorophores that have narrower, more closely spaced excitation and emission peaks, like BODIPY.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
Filter sizeOlympus 'Large' (FX)
SizeOlympus large-size (FX) set for U-M710I
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
Fluorophore5-FAM
Alexa Fluor 488
BODIPY
Calcein
FITC


FITC-2024B-U02

  • Size: Olympus large-size (FX) set for U-M710I

FITC-2024B-U02 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF02-485/20-32 Single Band Exciter 32 x 5.0 mm 1
FF01-524/24-32 Single Band Emitter 32 x 3.5 mm 1
FF506-Di03-32×44-FX Single Band Dichroic 32 x 44 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-524_24_Spectrum
FF02-485_20_spectrum
FF506-Di03_Spectrum
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FITC-2024B

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