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BrightLine® single-band filter set: DyLight 405 and similar fluorophores

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This filter set provides high brightness and contrast for imaging DyLight405™.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.

Physical Specifications (for standard parts - please contact us for custom sizes)
SizeLeica 'large' set
Olympus large-size (FX) set for U-M710I
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.

FluorophoreDyLight 405


  • Size: Leica 'large' set
  • Filter size: Leica 'Large'

DyLight405-C-L01 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF02-379/34-21.8-DSingle Band Exciter21.8 x 2.0 mm1
FF01-440/40-23.3-DSingle Band Emitter23.3 x 2.0 mm1
FF409-Di03-22x29Single Band Dichroic22.0 x 29.0 x 1.1 mm1


  • Size: Olympus large-size (FX) set for U-M710I
  • Filter size: Olympus 'Large' (FX)

DyLight405-C-U02 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF02-379/34-32Single Band Exciter32 x 5.0 mm1
FF01-440/40-32Single Band Emitter32 x 3.5 mm1
FF409-Di03-32x44-FXSingle Band Dichroic32 x 44 x 1.1 mm1


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Optical Density

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Semrock Spectral Plot Info

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Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.