Laser 2000
Home > Products > Light Delivery & Control > Microscopy Filters > Filter Sets & Cubes > Single-Band Microscopy Sets > BrightLine single-band filter set: Brilliant™ Violet 421 and similar fluorophores

BrightLine single-band filter set: Brilliant™ Violet 421 and similar fluorophores

Semrock Logo

This filter set is ideal for use with Brilliant™ Violet 421 and a variety of other blue fluorophores. It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio. Are you co-labeling with BUV395, BV510? This filter set is optimized to minimize crosstalk with BUV395 and BV510.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Olympus large-size (FX) set for U-M710I
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreAlexa Fluor 405
BFP (EBFP)
BV421 - BD Horizon Brilliant Violet 421
Cascade Blue
DyLight 405
HiLyte Fluor 405
LysoSensor Blue
LysoTracker Blue
Vybrant DyeCycle Violet


BV421-3824A-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard
  • Zero pixel shift: no

BV421-3824A-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-389/38-25 Single Band Exciter 25 x 5.0 mm 1
FF01-433/24-25 Single Band Emitter 25 x 3.5 mm 1
FF414-Di01-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

BV421-3824A-000-ZERO

  • Filter size: Standard
  • Zero pixel shift: yes

BV421-3824A-000-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-389/38-25 Single Band Exciter 25 x 5.0 mm 1
FF01-433/24-25 Single Band Emitter 25 x 3.5 mm 1
FF414-Di01-25×36 Single Band Dichroic 25.2 x 35.6 x 1.1 mm 1

BV421-3824A-L01-ZERO

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’
  • Zero pixel shift: yes

BV421-3824A-L01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-389/38-21.8-D Single Band Exciter 21.8 x 2.0 mm 1
FF01-433/24-23.3-D Single Band Emitter 23.3 x 2.0 mm 1
FF414-Di01-22×29 Single Band Dichroic 22.0 x 29.0 x 1.1 mm 1

BV421-3824A-U02

  • Size: Olympus large-size (FX) set for U-M710I
  • Filter size: Olympus ‘Large’ (FX)
  • Zero pixel shift: no

BV421-3824A-U02 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-389/38-32 Single Band Exciter 32 x 5.0 mm 1
FF01-433/24-32 Single Band Emitter 32 x 3.5 mm 1
FF414-Di01-32×44-FX Single Band Dichroic 32 x 44 x 1.1 mm 1

Transmission

Loading chart data...

Optical Density

Loading chart data...

Semrock Spectral Plot Info
FF01-389_38_Spectrum
FF01-433_24_Spectrum
FF414-Di01_Spectrum
BACK
TO TOP

Request a Demo

BV421-3824A

Ask the Expert

If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.