Laser 2000
Home > Products > Light Delivery & Control > Microscopy Filters > Filter Sets & Cubes > All Microscopy Filter Sets > BrightLine®; Sedat filter set: DAPI, FITC, & Texas Red and similar fluorophores, illuminated with LED-based light engines

BrightLine®; Sedat filter set: DAPI, FITC, & Texas Red and similar fluorophores, illuminated with LED-based light engines

Semrock Logo

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 7-filter ‘Sedat’ quad-band set is designed for high-speed, sequential imaging of DAPI, FITC, and Texas Red (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines, including 365 nm DAPI LEDs, compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter with three single-band emitters intended to be mounted in filter wheels and three single-band exciters which can be installed into the LED light engine or mounted in filter wheels.

Interested in learning how this filter set will perform with your light source?

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreDAPI
FITC
GFP
mCherry
Texas Red


LED-DA/FI/TX-3X3M-B-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

LED-DA/FI/TX-3X3M-B-000 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF01-378/52-25Single Band Exciter25 x 5.0 mm1
FF01-474/27-25Single Band Exciter25 x 5.0 mm1
FF03-575/25-25Single Band Exciter25 x 5.0 mm1
FF01-432/36-25Single Band Emitter25 x 3.5 mm1
FF02-525/40-25Single Band Emitter25 x 5.0 mm1
FF02-641/75-25Single Band Emitter25 x 3.5 mm1
FF409/493/596-Di02-25x36Triple Band Dichroic25.2 x 35.6 x 1.1 mm1

LED-DA/FI/TX-3X3M-B-L01

  • Size: Leica 'large' set
  • Filter size: Leica 'Large'

LED-DA/FI/TX-3X3M-B-L01 is a filter set, comprised of:

Product CodeFilter RoleFilter SizeQty
FF01-378/52-25Single Band Exciter25 x 5.0 mm1
FF01-474/27-25Single Band Exciter25 x 5.0 mm1
FF03-575/25-25Single Band Exciter25 x 5.0 mm1
FF01-432/36-25Single Band Emitter25 x 3.5 mm1
FF02-525/40-25Single Band Emitter25 x 5.0 mm1
FF02-641/75-25Single Band Emitter25 x 3.5 mm1
FF409/493/596-Di02-22x29Triple Band Dichroic22.0 x 29.0 x 1.1 mm1

Transmission

Loading chart data...

Optical Density

Loading chart data...

Semrock Spectral Plot Info
FF02-525_40_Spectrum
FF01-474_27_Spectrum
FF02-641_75_Spectrum
FF02-575_25_Spectrum
FF01-378_52_Spectrum
FF01-432_36_Spectrum
FF409_493_596-Di02_Spectrum
BACK
TO TOP

Request a Demo

LED-DA/FI/TX-3X3M-B

Ask the Expert

If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.