Laser 2000
Home > Products > Light Delivery & Control > Microscopy Filters > Filter Sets & Cubes > Single-Band Microscopy Sets > BrightLine long-pass filter set: DAPI and similar fluorophores
10-Year Warranty
30-Day Sale or Return
10% Academic Discount on Standard Semrock Optical Filters
Custom Sizing
Custom Coating

BrightLine long-pass filter set: DAPI and similar fluorophores

Semrock Logo

This unique ‘flamethrower’ filter set is recommended for those special cases in which it is critical to achieve the highest brightness for the shortest possible exposure times, or for very low fluorophore concentrations. For most applications that require minimized photobleaching, we recommend our DAPI-11LP-A set. In all cases our filters are uniquely hard-coated, for ‘no burn-out’ performance where it is most needed.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
Filter sizeOlympus 'Large' (FX)
SizeOlympus large-size (FX) set for U-M710I
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreAlexa Fluor 350
AMCA
BFP
DAPI
Fluoro-Gold
Hoechst 33258
Hoechst 33342
Hoechst 34580
LysoSensor Blue
Marina Blue
Pacific Blue
sgBFP


DAPI-50LP-A-U02

  • Size: Olympus large-size (FX) set for U-M710I

DAPI-50LP-A-U02 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-377/50-32 Single Band Exciter 32 x 5.0 mm 1
FF02-409/LP-32 Long Pass Emitter 32 x 3.5 mm 1
FF409-Di03-32×44-FX Single Band Dichroic 32 x 44 x 1.1 mm 1

Transmission

Loading chart data...

Optical Density

Loading chart data...

Semrock Spectral Plot Info
FF02-409_LP_Spectrum
FF01-377_50_spectrum
FF409-Di03_Spectrum

* Time Limited Offer  |  Terms & Conditions Apply  |   Contact us for Further Details

BACK
TO TOP

Request a Demo

DAPI-50LP-A

Ask the Expert

If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.