BrightLine® FRET filter set: Green & Red Fluorescent Protein and similar fluorophores

This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for green donor fluorescence and red acceptor fluorescence. FRET measurements using wide-field micro

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Specifications
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.

Physical Specifications (for standard parts - please contact us for custom sizes)
Size	Standard size (fits most microscopes) Leica 'large' set Sutter threaded set
Surface Quality (Scratch-Dig)	60-40
Exciter/Emitter Orientation	Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation	Reflective coating side should face toward light source and sample.

Fluorophores
Fluorophore	Alexa Fluor 488 Cy2 DsRed dTomato EGFP FITC GFP mCherry mStrawberry TRITC

FRET-GFP/RFP-D-000

Size: Standard size (fits most microscopes)
Filter size: Standard
Zero pixel shift: no

FRET-GFP/RFP-D-000 is a filter set, comprised of:

Product Code	Filter Role	Filter Size	Qty
FF02-472/30-25	Single Band Exciter	25 x 5.0 mm	1
FF01-520/35-25	Single Band Emitter	25 x 3.5 mm	1
FF02-641/75-25	Single Band Emitter	25 x 3.5 mm	1
FF495-Di03-25×36	Single Band Dichroic	25.2 x 35.6 x 1.1 mm	1

FRET-GFP/RFP-D-000-ZERO

Filter size: Standard
Zero pixel shift: yes

FRET-GFP/RFP-D-000-ZERO is a filter set, comprised of:

Product Code	Filter Role	Filter Size	Qty
FF02-472/30-25	Single Band Exciter	25 x 5.0 mm	1
FF01-520/35-25	Single Band Emitter	25 x 3.5 mm	1
FF02-641/75-25	Single Band Emitter	25 x 3.5 mm	1
FF495-Di03-25×36	Single Band Dichroic	25.2 x 35.6 x 1.1 mm	1

FRET-GFP/RFP-D-L01-ZERO

Size: Leica ‘large’ set
Filter size: Leica ‘Large’
Zero pixel shift: yes

FRET-GFP/RFP-D-L01-ZERO is a filter set, comprised of:

Product Code	Filter Role	Filter Size	Qty
FF02-472/30-21.8-D	Single Band Exciter	21.8 x 2.0 mm	1
FF01-520/35-25	Single Band Emitter	25 x 3.5 mm	1
FF02-641/75-25	Single Band Emitter	25 x 3.5 mm	1
FF495-Di03-22×29	Single Band Dichroic	22.0 x 29.0 x 1.1 mm	1

FRET-GFP/RFP-D-S01

Size: Sutter threaded set
Filter size: Sutter Threaded
Zero pixel shift: no

FRET-GFP/RFP-D-S01 is a filter set, comprised of:

Product Code	Filter Role	Filter Size	Qty
FF02-472/30-25	Single Band Exciter	25 x 5.0 mm	1
FF01-520/35-25-STR	Single Band Emitter	25 mm	1
FF02-641/75-25-STR	Single Band Emitter	25 mm	1
FF495-Di03-25×36	Single Band Dichroic	25.2 x 35.6 x 1.1 mm	1

FRET-GFP/RFP-D-S01-ZERO

Filter size: Sutter Threaded
Zero pixel shift: yes

FRET-GFP/RFP-D-S01-ZERO is a filter set, comprised of:

Product Code	Filter Role	Filter Size	Qty
FF02-472/30-25	Single Band Exciter	25 x 5.0 mm	1
FF01-520/35-25-STR	Single Band Emitter	25 mm	1
FF02-641/75-25-STR	Single Band Emitter	25 mm	1
FF495-Di03-25×36	Single Band Dichroic	25.2 x 35.6 x 1.1 mm	1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-520_35_spectrum
FF02-472_30_spectrum
FF495-Di03_Spectrum
FF02-641_75_Spectrum

Contact Laser 2000

Contact Laser 2000