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BrightLine Pinkel filter set: DAPI, FITC, TRITC, & Cy5 and similar fluorophores

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This 6-filter ‘Pinkel’ quad-band set is designed for sequentially imaging DAPI, FITC, TRITC, and Cy5 (and other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in quad-band performance, and has been tested by independent researchers to have up to four times the brightness, twice the signal-to-noise ratio and substantially lower crosstalk than any other such quad-band filter set on the market. The complete set is comprised of a quad-band beamsplitter and a quad-band emitter (these can be offered assembled in a microscope cube), plus four ‘no burn-out’ single-band exciters that are intended to be mounted in an exciter filter wheel or slider.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Sutter threaded set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreCy5
DAPI
FITC
GFP
TRITC


DA/FI/TR/Cy5-4X-B-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

DA/FI/TR/Cy5-4X-B-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-387/11-25 Single Band Exciter 25 x 5.0 mm 1
FF02-485/20-25 Single Band Exciter 25 x 5.0 mm 1
FF01-560/25-25 Single Band Exciter 25 x 5.0 mm 1
FF01-650/13-25 Single Band Exciter 25 x 5.0 mm 1
FF01-440/521/607/700-25 Quad Band Emitter 25 x 3.5 mm 1
FF410/504/582/669-Di01-25×36 Quad Band Dichroic 25.2 x 35.6 x 1.1 mm 1

DA/FI/TR/Cy5-4X-B-L01

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’

DA/FI/TR/Cy5-4X-B-L01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-387/11-25 Single Band Exciter 25 x 5.0 mm 1
FF02-485/20-25 Single Band Exciter 25 x 5.0 mm 1
FF01-560/25-25 Single Band Exciter 25 x 5.0 mm 1
FF01-650/13-25 Single Band Exciter 25 x 5.0 mm 1
FF01-440/521/607/700-23.3-D Quad Band Emitter 23.3 x 2.0 mm 1
FF410/504/582/669-Di01-22×29 Quad Band Dichroic 22.0 x 29.0 x 1.1 mm 1

DA/FI/TR/Cy5-4X-B-S01

  • Size: Sutter threaded set
  • Filter size: Sutter Threaded

DA/FI/TR/Cy5-4X-B-S01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-387/11-25-STR Single Band Exciter 25 mm 1
FF02-485/20-25-STR Single Band Exciter 25 mm 1
FF01-560/25-25-STR Single Band Exciter 25 mm 1
FF01-650/13-25-STR Single Band Exciter 25 mm 1
FF01-440/521/607/700-25 Quad Band Emitter 25 x 3.5 mm 1
FF410/504/582/669-Di01-25×36 Quad Band Dichroic 25.2 x 35.6 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-440_521_607_700_Spectrum
FF410_504_582_669-Di01_Spectrum
FF01-387_11_Spectrum
FF01-560_25_spectrum
FF01-650_13_spectrum
FF02-485_20_spectrum
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DA/FI/TR/Cy5-4X-B

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If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.

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