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BrightLine®; Pinkel filter set: DAPI, FITC, & Texas Red and similar fluorophores, illuminated with LED-based light engines

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Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 5-filter ‘Pinkel’ triple-band set is designed for imaging of a sample that is simultaneously labeled with DAPI, FITC, and Texas Red, (or other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines, including 365 nm DAPI LEDs, compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter and a triple-band emitter (these can be offered assembled in a microscope cube), plus three ‘no burn-out’ single-band exciters that are intended to be mounted in the LED-based light engines, or in an exciter filter wheel or slider.

Interested in learning how this filter set will perform with your light source?

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Sutter threaded set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreDAPI
FITC
GFP
mCherry
Texas Red


LED-DA/FI/TX-3X-B-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard
  • Zero pixel shift: no

LED-DA/FI/TX-3X-B-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-378/52-25 Single Band Exciter 25 x 5.0 mm 1
FF01-474/27-25 Single Band Exciter 25 x 5.0 mm 1
FF03-575/25-25 Single Band Exciter 25 x 5.0 mm 1
FF01-432/523/702-25 Triple Band Emitter 25 x 3.5 mm 1
FF409/493/596-Di02-25×36 Triple Band Dichroic 25.2 x 35.6 x 1.1 mm 1

LED-DA/FI/TX-3X-B-000-ZERO

  • Filter size: Standard
  • Zero pixel shift: yes

LED-DA/FI/TX-3X-B-000-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-378/52-25 Single Band Exciter 25 x 5.0 mm 1
FF01-474/27-25 Single Band Exciter 25 x 5.0 mm 1
FF03-575/25-25 Single Band Exciter 25 x 5.0 mm 1
FF01-432/523/702-25 Triple Band Emitter 25 x 3.5 mm 1
FF409/493/596-Di02-25×36 Triple Band Dichroic 25.2 x 35.6 x 1.1 mm 1

LED-DA/FI/TX-3X-B-L01-ZERO

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’
  • Zero pixel shift: yes

LED-DA/FI/TX-3X-B-L01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-378/52-25 Single Band Exciter 25 x 5.0 mm 1
FF01-474/27-25 Single Band Exciter 25 x 5.0 mm 1
FF03-575/25-25 Single Band Exciter 25 x 5.0 mm 1
FF01-432/523/702-23.3-D Triple Band Emitter 23.3 x 2.0 mm 1
FF409/493/596-Di02-22×29 Triple Band Dichroic 22.0 x 29.0 x 1.1 mm 1

LED-DA/FI/TX-3X-B-S01

  • Size: Sutter threaded set
  • Filter size: Sutter Threaded
  • Zero pixel shift: no

LED-DA/FI/TX-3X-B-S01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-378/52-25-STR Single Band Exciter 25 mm 1
FF01-474/27-25-STR Single Band Exciter 25 mm 1
FF03-575/25-25-STR Single Band Exciter 25 mm 1
FF01-432/523/702-25 Triple Band Emitter 25 x 3.5 mm 1
FF409/493/596-Di02-25×36 Triple Band Dichroic 25.2 x 35.6 x 1.1 mm 1

LED-DA/FI/TX-3X-B-S01-ZERO

  • Filter size: Sutter Threaded
  • Zero pixel shift: yes

LED-DA/FI/TX-3X-B-S01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-378/52-25-STR Single Band Exciter 25 mm 1
FF01-474/27-25-STR Single Band Exciter 25 mm 1
FF03-575/25-25-STR Single Band Exciter 25 mm 1
FF01-432/523/702-25 Triple Band Emitter 25 x 3.5 mm 1
FF409/493/596-Di02-25×36 Triple Band Dichroic 25.2 x 35.6 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-474_27_Spectrum
FF01-432_523_702_Spectrum
FF02-575_25_Spectrum
FF01-378_52_Spectrum
FF409_493_596-Di02_Spectrum
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LED-DA/FI/TX-3X-B

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If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.