BrightLine® Pinkel filter set: DAPI, FITC, TRITC, & Cy5 and similar fluorophores, illuminated with LED-based light engines

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 6-filter ‘Pinkel’ quad-band set is designed for imaging of a sample that is simultaneously labeled with DAPI, FITC, TRITC, and Cy5 (or other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines, including 365 nm DAPI LEDs, compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a quad-band beamsplitter and a quad-band emitter (these can be offered assembled in a microscope cube), plus four ‘no burn-out’ single-band exciters that are intended to be mounted in the LED-based light engines, or in an exciter filter wheel or slider.

Interested in learning how this filter set will perform with your light source?

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.