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Home > Products > Light Delivery & Control > Microscopy Filters > Filter Sets & Cubes > All Microscopy Filter Sets > BrightLine®; full-multiband filter set: DAPI, FITC, TRITC, Cy5, & Cy7 and similar fluorophores, illuminated with Lumencor CELESTA light engine

BrightLine®; full-multiband filter set: DAPI, FITC, TRITC, Cy5, & Cy7 and similar fluorophores, illuminated with Lumencor CELESTA light engine

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This Full-multiband penta-band set is designed for imaging DAPI, FITC, TRITC, Cy5 and Cy7 (and other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in penta-band performance, and has been optimized to be illuminated with Lumencor CELESTA light engine® utilizing 408 nm, 473 nm, 545 nm, 635 nm & 750 nm wavelengths. This set has been designed to deliver significant fluorescence signal improvements for the CELESTA light engine compared with sets designed for standard broadband light sources such as mercury or xenon arc lamps or other LED-based light engines. The complete set is comprised of a penta-band beamsplitter, emitter, and exciter filter, all with ‘no burn-out’ performance.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.


General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


Fluorophores
FluorophoreCy5
Cy7
DAPI
Deep
FITC
GFP
TRITC


CELESTA-DA/FI/TR/Cy5/Cy7-A-000

  • Size: Standard size (fits most microscopes)
  • Filter size: Standard
  • Zero pixel shift: no

CELESTA-DA/FI/TR/Cy5/Cy7-A-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-391/477/549/639/741-25 Penta Band Exciter 25 x 5.0 mm 1
FF01-441/511/593/684/817-25 Penta Band Emitter 25 x 3.5 mm 1
FF421/491/567/659/776-Di01-25×36 Penta Band Dichroic 25.2 x 35.6 x 1.1 mm 1

CELESTA-DA/FI/TR/Cy5/Cy7-A-000-ZERO

  • Filter size: Standard
  • Zero pixel shift: yes

CELESTA-DA/FI/TR/Cy5/Cy7-A-000-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-391/477/549/639/741-25 Penta Band Exciter 25 x 5.0 mm 1
FF01-441/511/593/684/817-25 Penta Band Emitter 25 x 3.5 mm 1
FF421/491/567/659/776-Di01-25×36 Penta Band Dichroic 25.2 x 35.6 x 1.1 mm 1

CELESTA-DA/FI/TR/Cy5/Cy7-A-L01-ZERO

  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’
  • Zero pixel shift: yes

CELESTA-DA/FI/TR/Cy5/Cy7-A-L01-ZERO is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-391/477/549/639/741-21.8-D Penta Band Exciter 21.8 x 3.5 mm 1
FF01-441/511/593/684/817-23.3-D Penta Band Emitter 23.3 x 2.0 mm 1
FF421/491/567/659/776-Di01-22×29 Penta Band Dichroic 22.0 x 29.0 x 1.1 mm 1

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-441_511_593_684_817_Spectrum
FF01-391_477_549_639_741_Spectrum
FF421_491_567_659_776-Di01__Spectrum
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CELESTA-DA/FI/TR/Cy5/Cy7-A

Ask the Expert

If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.