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Home > Products > Light Delivery & Control > Microscopy Filters > Filter Sets & Cubes > Full Multiband Sets > BrightLine full-multiband filter set: DAPI, FITC, & Texas Red and similar fluorophores

BrightLine full-multiband filter set: DAPI, FITC, & Texas Red and similar fluorophores

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Semrock’s award-winning blue-green-red multiband filter set is uniquely optimized to provide simultaneously stunning brightness, striking contrast, and superb color balance when viewing multiple fluorophores by eye or with a high-quality color CCD camera. The result is brilliant colors and a black background.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.

Physical Specifications (for standard parts - please contact us for custom sizes)
SizeStandard size (fits most microscopes)
Leica 'large' set
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.

FluorophoreAlexa Fluor 488
Alexa Fluor 568
Alexa Fluor 594
Hoechst 33258
Hoechst 33342
Hoechst 34580
MitoTracker Red
Texas Red


  • Size: Standard size (fits most microscopes)
  • Filter size: Standard

DA/FI/TX-B-000 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-407/494/576-25 Triple Band Exciter 25 x 5.0 mm 1
FF01-457/530/628-25 Triple Band Emitter 25 x 3.5 mm 1
FF436/514/604-Di01-25×36 Triple Band Dichroic 25.2 x 35.6 x 1.1 mm 1


  • Size: Leica ‘large’ set
  • Filter size: Leica ‘Large’

DA/FI/TX-B-L01 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-407/494/576-21.8-D Triple Band Exciter 21.8 x 2.0 mm 1
FF01-457/530/628-23.3-D Triple Band Emitter 23.3 x 2.0 mm 1
FF436/514/604-Di01-22×29 Triple Band Dichroic 22.0 x 29.0 x 1.1 mm 1


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Optical Density

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Semrock Spectral Plot Info

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Ask the Expert

If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.