3D Microscope Upgrade
Easily capture and analyse 3D images of cellular structures down to the single molecule level
- Single or dual channel. Up to four wavelengths with multi-colour masks
- Extends the capabilities of your microscope for nano-scale 3D imaging and sensing
- Attaches between any widefield microscope and EMCCD or sCMOS camera, using standard C-mounts
- Ensures pupil plane alignment to your microscope and objectives with corrective optics
- Features a bypass mode so you can revert to 2D imaging without having to dismantle
- Unlocks vast potential when used with other tools and techniques, including SOFI, confocal, STORM, widefield, fluorescent beads, dyes, and photoactivable proteins
Unrivalled Precision and Depth 3D Imaging & Tracking
Double Helix Optics’ SPINDLE™ gives researchers the ability to easily capture and analyse 3D images of cellular structures down to the single molecule level.
Using Double Helix Optics’ patented Light Engineering™ technology as its foundation, the SPINDLE™ can be easily installed on existing microscopes to enable advanced 3D imaging and tracking with super-resolution capabilities. Built-in bypass mode allows for easy return to non-3D experiments.
- Patented phase mask design overcomes traditional limitations enabling 3D imaging with unparalleled depth and axial precision
- Select from a library of masks optimised to the emission wavelength needed for your 3D experiment
- Compatible with wide range of microscopes, objectives and cameras
Custom designed optics for precision imaging and tracking
- Full field-of-view imaging
- Custom optics ensure diffraction-limited performance over the full field of view of large format sensors
- Transmission >95%
- Built-in corrective optics to ensure pupil plane alignment to your microscope and objectives
- Ease of install with stable alignment of x, y, and z positions of the phase mask in the relayed pupil plane
Intelligent data analysis
- 3DTRAX™ Software, a FIJI plugin provides: 3D localisation of molecules, 3D rendering, drift correction, tracking, visualisation
- Intuitive plots help to ensure quality data throughout the analysis process
- Quantitative analysis
- Ease of file export for extended analysis
Affordable and adaptable
- Small footprint allows easy installation even in space-constrained environments
- Input and output C-mount adapters provide easy support for commercial and custom-built microscopes and cameras
- Highly reliable system with no moving parts. Switchable phase mask cartridges, auxiliary emission filter holders for maximum experiment flexibility
- Modular design evolves your existing system into an advanced 3D imaging system with super-resolution capabilities
2019 Prism Award Winner
Going up against innovative technologies at SPIE’s Photonics West, the SPINDLE™ secured the win for Double Helix Optics for the category of diagnostics and therapeutics. Click here to read SPIE’s press release on the 2019 Prism Award winners.
