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738 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for standard microscopy

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BrightLine® image-splitting dichroic beamsplitters offer superb image quality for both transmitted and reflected light when separating beams of light by color for simultaneous capture of multi-color images. For applications such as (FRET) and real-time live-cell imaging, users can now separate two, four or even more colors onto as many cameras or regions of a single camera sensor. The exceptional flatness of these filters virtually eliminates aberrations in the reflected beam for most common imaging systems.

Common Fluorophore Pair to Split: Cy5.5/Cy7

FF738-FDi01-t1 image-splitting dichroic beamsplitter for standard micrscopy available on 1 mm substrate for reflecting imaging beams up to 10 mm in diameter while minimizing RWE

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
Transmission BandTavg > 93% 754 – 1200 nm
Reflection BandRavg > 95% 400 – 722 nm
Edge Wavelength738 nm
Common Fluorophore Pair to SplitCy5.5/Cy7


General Specifications
Angle of Incidence45 ± 1.5 °
Cone Half-angle2 °
SteepnessStandard
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.
Flatness / RWE ClassificationImage-splitting
Reflected Wavefront Error< 0.2λ P-V RWE @ 632.8


Physical Specifications (for standard parts - please contact us for custom sizes)
Mounted / UnmountedUnmounted
ShapeRectangular
Size25.2 x 35.6 x 1.1 mm
25.2 x 35.6 x 3.0 mm
Transverse Dimensions, LxW25.2 mm x 35.6 mm
Transverse Tolerance, Mounted± 0.1 mm
Filter Thickness, Unmounted3.0 mm
Clear Aperture≥ 80% (elliptical)
Surface Quality (Scratch-Dig)60-40
Substrate TypeLow-autofluorescence optical quality glass


FF738-FDi01-t1-25×36

FF738-FDi01-t3-25×36


Transmission

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Optical Density

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Semrock Spectral Plot Info
FF738-FDi01-t1_Spectrum
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FF738-FDi01

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