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699 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for standard microscopy

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BrightLine® image-splitting dichroic beamsplitters offer superb image quality for both transmitted and reflected light when separating beams of light by color for simultaneous capture of multi-color images. For applications such as (FRET) and real-time live-cell imaging, users can now separate two, four or even more colors onto as many cameras or regions of a single camera sensor. The exceptional flatness of these filters virtually eliminates aberrations in the reflected beam for most common imaging systems.

Semrock’s image-splitting dichroics for super-resolution microscopy deliver industry-leading λ/5 P-V RWE on 3 mm thick substrates for minimal focus shift and optical wavefront aberrations of the reflected imaging beam. These dichroics can be used with much larger diameter imaging beams up to 37 mm while minimizing RWE in custom size versions.

Common Fluorophore Pair to Split: iRFP670/iRFP720 or AlexaFluor647/AlexaFluor700

FF699-FDi01-t1 image-splitting dichroic beamsplitter for standard micrscopy available on 1 mm substrate for reflecting imaging beams up to 10 mm in diameter while minimizing RWE

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
Transmission BandTavg > 93% 712 – 1200 nm
Reflection BandRavg > 95% 350 – 686 nm
Edge Wavelength699 nm
Common Fluorophore Pair to SplitIRFP670/iRFP720 or AlexaFluor647/AlexaFluor700


General Specifications
Angle of Incidence45 ± 1.5 °
Cone Half-angle2 °
SteepnessStandard
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.
Flatness / RWE ClassificationImage-splitting


Physical Specifications (for standard parts - please contact us for custom sizes)
Mounted / UnmountedUnmounted
ShapeRectangular
Size25.2 x 35.6 x 1.1 mm
25.2 x 35.6 x 3.0 mm
Transverse Dimensions, LxW25.2 mm x 35.6 mm
Transverse Tolerance, Mounted± 0.1 mm
Filter Thickness, Unmounted3.0 mm
Filter Thickness Tolerance, Unmounted± 0.1 mm
Clear Aperture≥ 80% (elliptical)
Surface Quality (Scratch-Dig)60-40
Substrate Thickness3.0 mm
Substrate TypeLow-autofluorescence optical quality glass


FF699-FDi01-t1-25×36

FF699-FDi01-t3-25×36

  • Filter Effective Index: 1.91 Understanding ‘Effective Index of Refraction’ neff
  • Orientation: Reflective surface marked with laser dot – Orient in direction of incoming light
  • Reflected Wavefront Error: < 0.2λ P-V RWE @ 632.8 @45°
    < 2λ P-V RWE @ 632.8
  • Size: 25.2 x 35.6 x 3.0 mm

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF699-FDi01-t1_Spectrum
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FF699-FDi01

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