536/40 nm BrightLine® single-band bandpass filter

Individual fluorescence bandpass filters that have been optimized for use in a variety of fluorescence instruments. All thin-film, hard-coated construction for unsurpassed performance and reliability.

Optical Specifications
Transmission Band	T_avg > 93% 516 – 556 nm
Transmission Wavelength	536 nm
Transmission Wavelength Range	516 – 556 nm
FWHM Bandwidth	45.3 nm
Blocking Band	OD_avg > 1.5 200 – 400 nm OD_avg > 2 400 – 425 nm OD_avg > 5 425 – 457 nm OD_avg > 10 457 – 497 nm (Design specification – measurements are noise-floor limited) OD > 4.5 506 nm OD_avg > 5 569 – 700 nm OD_avg > 5 700 – 925 nm

General Specifications
Angle of Incidence	0 ± 5 °
Cone Half-angle	7 °
Filter Effective Index	1.83 Understanding ‘Effective Index of Refraction’ n_eff
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.

Physical Specifications (for standard parts – please contact us for custom sizes)
Transverse Dimensions, Diameter	25 mm
Filter Thickness, Mounted	3.5 mm
Filter Thickness Tolerance, Mounted	± 0.1 mm
Filter Thickness, Unmounted	2.0 mm
Filter Thickness Tolerance, Unmounted	± 0.1 mm
Clear Aperture	≥ 22 mm
Surface Quality (Scratch-Dig)	60-40
Orientation	Arrow on ring is preferred direction of propagation of light

Fluorophores
Fluorophore	5-FAM (5-carboxyfluorescein) SYBR Green I nucleic acid gel stain-DNA

FF01-536/40-25

Mounted
Size: 25 x 3.5 mm

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-536_40_spectrum

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