Laser 2000

535/40 Avant™ Bandpass Filter, 25 mm

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For optimal filter set performance, pair this exciter with AF01-584/40 and AF560-Di01, which have been optimized for Cy3™, Alexa Fluor 555, and other key fluorophores. Precise edge placement of the steep spectral edges combined with deep complementary blocking maximize fluorophore performance compared to standard exciter and emitter pairs.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
Transmission BandTavg > 93% 515 – 555 nm
Transmission Wavelength535 nm
FWHM Bandwidth43.5 nm
Blocking BandODavg > 5 200 – 275 nm (Design specification – measurements are noise-floor limited)
ODavg > 5 275 – 340 nm
ODavg > 6 340 – 503 nm
ODabs > 3.5 @ 559.5 nm
ODavg > 10 564 – 618 nm (Design specification – measurements are noise-floor limited)
ODavg > 6 618 – 850 nm
ODavg > 2 850 – 1100 nm

General Specifications
Angle of Incidence0 ± 5 °
Cone Half-angle7 °
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.

Physical Specifications (for standard parts - please contact us for custom sizes)
Mounted / UnmountedMounted
Size25 x 5.0 mm
Transverse Dimensions, Diameter25 mm
Transverse Tolerance, Mounted+ 0.0 / - 0.1 mm
Substrate Thickness Tolerance, Unmounted± 0.1 mm
Filter Thickness, Mounted5 mm
Filter Thickness Tolerance, Mounted± 0.1 mm
Clear Aperture≥ 21 mm
Surface Quality (Scratch-Dig)60-40
Substrate Thickness2.0 mm
Substrate TypeLow-autofluorescence optical quality glass
OrientationArrow on ring indicates preferred direction of propagation of light


  • Size: 25 x 5.0 mm


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Optical Density

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Semrock Spectral Plot Info

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