534/30 nm BrightLine® single-band bandpass filter

Individual fluorescence bandpass filters that have been optimized for use in a variety of fluorescence instruments. All thin-film, hard-coated construction for unsurpassed performance and reliability.

Optical Specifications
Transmission Band	T_avg > 90% 519.5 – 549.5 nm
Transmission Wavelength	534.5 nm
Transmission Wavelength Range	519 – 549 nm
FWHM Bandwidth	37.7 nm
Blocking Band	OD_avg > 4 1000 – 1200 nm OD_avg > 5.5 300 – 475.5 nm OD_avg > 12 475.5 – 495.5 nm (Design specification – measurements are noise-floor limited). OD_abs > 6 495.5 – 510.5 nm OD_avg > 5.5 563 – 800 nm ODavg > 5 800 – 1000 nm

General Specifications
Angle of Incidence	0 ± 5 °
Cone Half-angle	7 °
Filter Effective Index	1.81 Understanding ‘Effective Index of Refraction’ n_eff
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.

Physical Specifications (for standard parts – please contact us for custom sizes)
Transverse Dimensions, Diameter	25 mm
Filter Thickness, Mounted	5.0 mm
Filter Thickness Tolerance, Mounted	± 0.1 mm
Filter Thickness, Unmounted	2.0 mm
Filter Thickness Tolerance, Unmounted	± 0.1 mm
Clear Aperture	≥ 21 mm
Surface Quality (Scratch-Dig)	60-40
Orientation	Arrow on ring is preferred direction of propagation of light

Fluorophores
Fluorophore	5-FAM (5-carboxyfluorescein) 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) SYBR Green I nucleic acid gel stain-DNA

FF02-534/30-25

Mounted
Size: 25 x 5.0 mm

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF02-534_30_spectrum

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