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494/20 nm BrightLine® single-band bandpass filter

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Individual fluorescence bandpass filters that have been optimized for use in a variety of fluorescence instruments. All thin-film, hard-coated construction for unsurpassed performance and reliability.

Optical Specifications
Transmission Band Tavg > 93% 484 – 504 nm

Transmission Wavelength 494 nm

Transmission Wavelength Range 484 – 504 nm

FWHM Bandwidth 25.2 nm

Blocking Band ODavg > 6 200 – 275 nm (Design specification – measurements are noise-floor limited).
ODavg > 6 275 – 375 nm
ODavg > 10 375 – 468 nm (Design specification – measurements are noise-floor limited).
ODavg > 10 516 – 650 nm (Design specification – measurements are noise-floor limited)
ODavg > 5 650 – 700 nm
ODavg > 2.5 700 – 925 nm
ODavg > 2 925 – 1150 nm
General Specifications
Angle of Incidence 0 ± 5 °

Cone Half-angle 7 °

Filter Effective Index 1.89 Understanding ‘Effective Index of Refraction’ neff

Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.
Physical Specifications (for standard parts – please contact us for custom sizes)
Transverse Dimensions, Diameter 25 mm

Filter Thickness, Mounted 5.0 mm

Filter Thickness Tolerance, Mounted ± 0.1 mm

Filter Thickness, Unmounted 2.0 mm

Filter Thickness Tolerance, Unmounted ± 0.1 mm

Clear Aperture ≥ 21 mm

Surface Quality (Scratch-Dig) 60-40

Orientation Arrow on ring is preferred direction of propagation of light
Fluorophores
Fluorophore 5-FAM (5-carboxyfluorescein)
SYBR Green I nucleic acid gel stain-DNA

FF01-494/20-25

  • Mounted
  • Size: 25 x 5.0 mm

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-494_20_Spectrum

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