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446/510/581/703 nm BrightLine® quad-band bandpass filter

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Individual multiband fluorescence bandpass filters that utilize Semrock’s patented single-substrate construction. These filters have extremely high transmission, steep and well-defined edges, and outstanding blocking between the passbands. All thin-film, hard-coated construction for unsurpassed performance and reliability.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
Transmission BandBand 1: Tavg > 93% 429.5 – 462.0 nm
Band 2: Tavg > 93% 502.5 – 518.5 nm
Band 3: Tavg > 93% 550 – 613 nm
Band 4: Tavg > 93% 663 – 743 nm
Transmission WavelengthBand 1: 445.8 nm
Band 2: 510.5 nm
Band 3: 581.5 nm
Band 4: 703 nm
Transmission Wavelength Range663 - 743 nm
FWHM BandwidthBand 2: 20.5 nm
Band 1: 37.4 nm
Band 3: 69.3 nm
Band 4: 87.6 nm
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
Blocking BandOD > 3.5 419.8 nm
OD > 3.5 467.5 nm
OD > 3.5 496.8 nm
OD > 3.5 524.5 nm
OD > 3.5 541.8 nm
OD > 3.5 622.9 nm
OD > 3.5 655.1 nm
ODavg > 4 200 – 370 nm
ODavg > 5 1064 nm
ODavg > 5 760.9 – 900 nm
ODavg > 8 370 – 410 nm (Design specification – measurements are noise-floor limited)
ODavg > 8 473 – 491 nm (Design specification – measurements are noise-floor limited)
ODavg > 8 530.5 – 533.5 nm (Design specification – measurements are noise-floor limited)
ODavg > 8 632.8 – 647.1 nm (Design specification – measurements are noise-floor limited)


General Specifications
Angle of Incidence0 ± 5 °
Cone Half-angle7 °
Filter Effective Index1.99 Understanding 'Effective Index of Refraction' neff
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
ShapeRound
Size25 x 3.5 mm
23.3 x 2.0 mm
Transverse Dimensions, Diameter25 mm
Transverse Tolerance, Mounted+ 0.0 / - 0.1 mm
Filter Thickness, Mounted3.5 mm
Filter Thickness Tolerance, Mounted± 0.1 mm
Clear Aperture≥ 22 mm
Surface Quality (Scratch-Dig)60-40
Substrate Thickness2.0 mm
Substrate TypeLow-autofluorescence optical quality glass
OrientationArrow on ring indicates preferred direction of propagation of light
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.


FF01-446/510/581/703-23.3-D

  • Unmounted
  • Size: 23.3 x 2.0 mm

FF01-446/510/581/703-25

  • Mounted
  • Size: 25 x 3.5 mm

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-446_510_581_703_Spectrum
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FF01-446/510/581/703

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Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.

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Alternatively, just call +44 (0) 1933 461 666 and ask to speak to Toby.

Toby Scrivener

MPhys (Hons)

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Karen McGeachy West

MPhys (Hons)

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Peter Collins

Peter Collins

BEng (Hons), MSc (Eng)

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Siân Brooks

BSc (Hons)

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Dan Young

BSc (Hons)

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Paul Williams

PhD

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Lisa Thomson

MPhys

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Shannon O’Connor

BSc (Hons)