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391/477/549/639/741 nm BrightLine® penta-band bandpass filter

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Individual multiband fluorescence bandpass filters that utilize Semrock’s patented single-substrate construction. These filters have extremely high transmission, steep and well-defined edges, and outstanding blocking between the passbands. All thin-film, hard-coated construction for unsurpassed performance and reliability.

BrightLine exciter for DAPI/FITC/TRITC/Cy5/Cy7 imaging channels optimized for Lumencor CELESTA light engine® utilizing 408 nm, 473 nm, 545 nm, 635 nm & 750 nm wavelengths.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
Transmission BandBand 1: Tavg > 85% 369 – 413 nm
Band 2: Tavg > 90% 471 – 483 nm
Band 3: Tavg > 90% 541 – 557 nm
Band 4: Tavg > 90% 630 – 647 nm
Band 5: Tavg > 90% 725 – 757 nm
Transmission WavelengthBand 1: 391 nm
Band 2: 477 nm
Band 3: 549 nm
Band 4: 638.5 nm
Band 5: 741 nm
FWHM BandwidthBand 2: 20.4 nm
Band 3: 25.7 nm
Band 4: 27.6 nm
Band 5: 45.1 nm
Band 1: 54.4 nm
Blocking BandOD > 3.5 420 nm
OD > 3.5 463 nm
OD > 3.5 491 nm
OD > 3.5 532 nm
OD > 3.5 566 nm
OD > 3.5 620 nm
OD > 3.5 657.5 nm
OD > 3.5 712 nm
OD > 3.5 771 nm
ODavg > 2 925 – 1100 nm
ODavg > 4 850 – 925 nm
ODavg > 6 200 – 275 nm (Design specification – measurements are noise-floor limited)
ODavg > 6 275 – 358 nm
ODavg > 6 426 – 456 nm
ODavg > 6 498 – 524 nm
ODavg > 6 574 – 611 nm
ODavg > 6 667 – 701 nm
ODavg > 6 784 – 850 nm


General Specifications
Angle of Incidence0 ± 5 °
Cone Half-angle7 °
Filter Effective Index1.81 Understanding 'Effective Index of Refraction' neff
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
ShapeRound
Size25 x 5.0 mm
21.8 x 3.5 mm
Transverse Dimensions, Diameter25 mm
Transverse Tolerance, Mounted+ 0.0 / - 0.1 mm
Filter Thickness, Mounted5.0 mm
Filter Thickness Tolerance, Mounted± 0.1 mm
Clear Aperture≥ 21 mm
Surface Quality (Scratch-Dig)60-40
Substrate Thickness3.5 mm
Substrate TypeLow-autofluorescence optical quality glass
OrientationArrow on ring indicates preferred direction of propagation of light


FF01-391/477/549/639/741-21.8-D

  • Unmounted
  • Size: 21.8 x 3.5 mm

FF01-391/477/549/639/741-25

  • Mounted
  • Size: 25 x 5.0 mm

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-391_477_549_639_741_Spectrum
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FF01-391/477/549/639/741

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Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.

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Karen McGeachy West

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Peter Collins

BEng (Hons), MSc (Eng)

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Shannon O’Connor

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