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391/477/549/639/741 nm BrightLine® penta-band bandpass filter

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Individual multiband fluorescence bandpass filters that utilize Semrock’s patented single-substrate construction. These filters have extremely high transmission, steep and well-defined edges, and outstanding blocking between the passbands. All thin-film, hard-coated construction for unsurpassed performance and reliability.

BrightLine exciter for DAPI/FITC/TRITC/Cy5/Cy7 imaging channels optimized for Lumencor CELESTA light engine® utilizing 408 nm, 473 nm, 545 nm, 635 nm & 750 nm wavelengths.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
Transmission BandBand 1: Tavg > 85% 369 – 413 nm
Band 2: Tavg > 90% 471 – 483 nm
Band 3: Tavg > 90% 541 – 557 nm
Band 4: Tavg > 90% 630 – 647 nm
Band 5: Tavg > 90% 725 – 757 nm
Transmission WavelengthBand 1: 391 nm
Band 2: 477 nm
Band 3: 549 nm
Band 4: 638.5 nm
Band 5: 741 nm
FWHM BandwidthBand 2: 20.4 nm
Band 3: 25.7 nm
Band 4: 27.6 nm
Band 5: 45.1 nm
Band 1: 54.4 nm
Blocking BandOD > 3.5 420 nm
OD > 3.5 463 nm
OD > 3.5 491 nm
OD > 3.5 532 nm
OD > 3.5 566 nm
OD > 3.5 620 nm
OD > 3.5 657.5 nm
OD > 3.5 712 nm
OD > 3.5 771 nm
ODavg > 2 925 – 1100 nm
ODavg > 4 850 – 925 nm
ODavg > 6 200 – 275 nm (Design specification – measurements are noise-floor limited)
ODavg > 6 275 – 358 nm
ODavg > 6 426 – 456 nm
ODavg > 6 498 – 524 nm
ODavg > 6 574 – 611 nm
ODavg > 6 667 – 701 nm
ODavg > 6 784 – 850 nm


General Specifications
Angle of Incidence0 ± 5 °
Cone Half-angle7 °
Filter Effective Index1.81 Understanding 'Effective Index of Refraction' neff
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.


Physical Specifications (for standard parts - please contact us for custom sizes)
ShapeRound
Size25 x 5.0 mm
21.8 x 3.5 mm
Transverse Dimensions, Diameter25 mm
Transverse Tolerance, Mounted+ 0.0 / - 0.1 mm
Filter Thickness, Mounted5.0 mm
Filter Thickness Tolerance, Mounted± 0.1 mm
Clear Aperture≥ 21 mm
Surface Quality (Scratch-Dig)60-40
Substrate Thickness3.5 mm
Substrate TypeLow-autofluorescence optical quality glass
OrientationArrow on ring indicates preferred direction of propagation of light


FF01-391/477/549/639/741-21.8-D

  • Unmounted
  • Size: 21.8 x 3.5 mm

FF01-391/477/549/639/741-25

  • Mounted
  • Size: 25 x 5.0 mm

Transmission

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Optical Density

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Semrock Spectral Plot Info
FF01-391_477_549_639_741_Spectrum
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FF01-391/477/549/639/741

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If you’re interested in the products shown here, or have questions about something you can’t find on our website, please get in touch through the form below.

Our knowledgeable sales team will be happy to help. We can normally source exactly what you need, or work with you to come up with custom solutions.

Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.

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