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BrightLine single-band filter set: TRITC and similar fluorophores

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This filter set is ideal for use with TRITC and a number of Rhodamine dyes, as well as other orange fluorophores. This improved set offers the high brightness that has made Semrock famous, and yet with its narrow exciter filter, it also provides a high signal-to-noise ratio and minimizes the bleedthrough in co-labeling applications that is associated with shorter-wavelength yellow and green fluorescent markers, like yellow fluorescent protein (YFP), green fluorescent protein (GFP), and FITC. Note that the narrow excitation filter efficiently captures the 546 nm emission line of mercury or metal-halide-mercury arc lamps.

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hrs.

Physical Specifications (for standard parts - please contact us for custom sizes)
Filter sizeOlympus 'Large' (FX)
SizeOlympus large-size (FX) set for U-M710I
Surface Quality (Scratch-Dig)60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic OrientationReflective coating side should face toward light source and sample.

Alexa Fluor 532
Alexa Fluor 546


  • Size: Olympus large-size (FX) set for U-M710I

TRITC-B-U02 is a filter set, comprised of:

Product Code Filter Role Filter Size Qty
FF01-543/22-32 Single Band Exciter 32 x 5.0 mm 1
FF01-593/40-32 Single Band Emitter 32 x 3.5 mm 1
FF562-Di03-32×44-FX Single Band Dichroic 32 x 44 x 1.1 mm 1


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Optical Density

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Semrock Spectral Plot Info

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Alternatively, just call +44 (0) 1933 461 666 and ask to speak to the Photonics team.