Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.
This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring DAPI (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market. Interested in learning how this filter set will perform with your light source?
Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spectral plot data, please see here for more information.
LED-DAPI-B-000
- Size: Standard size (fits most microscopes)
- Filter size: Standard
- Zero pixel shift: no
LED-DAPI-B-000 is a filter set, comprised of:
| Product Code |
Filter Role |
Filter Size |
Qty |
| FF01-378/52-25 |
Single Band Exciter |
25 x 5.0 mm |
1 |
| FF02-447/60-25 |
Single Band Emitter |
25 x 3.5 mm |
1 |
| FF409-Di03-25×36 |
Single Band Dichroic |
25.2 x 35.6 x 1.1 mm |
1 |
LED-DAPI-B-000-ZERO
- Filter size: Standard
- Zero pixel shift: yes
LED-DAPI-B-000-ZERO is a filter set, comprised of:
| Product Code |
Filter Role |
Filter Size |
Qty |
| FF01-378/52-25 |
Single Band Exciter |
25 x 5.0 mm |
1 |
| FF02-447/60-25 |
Single Band Emitter |
25 x 3.5 mm |
1 |
| FF409-Di03-25×36 |
Single Band Dichroic |
25.2 x 35.6 x 1.1 mm |
1 |
LED-DAPI-B-L01-ZERO
- Size: Leica ‘large’ set
- Filter size: Leica ‘Large’
- Zero pixel shift: yes
LED-DAPI-B-L01-ZERO is a filter set, comprised of:
| Product Code |
Filter Role |
Filter Size |
Qty |
| FF01-378/52-21.8-D |
Single Band Exciter |
21.8 x 2.0 mm |
1 |
| FF02-447/60-23.3-D |
Single Band Emitter |
23.3 x 2.0 mm |
1 |
| FF409-Di03-22×29 |
Single Band Dichroic |
22.0 x 29.0 x 1.1 mm |
1 |
LED-DAPI-B-U02
- Size: Olympus large-size (FX) set for U-M710I
- Filter size: Olympus ‘Large’ (FX)
- Zero pixel shift: no
LED-DAPI-B-U02 is a filter set, comprised of:
| Product Code |
Filter Role |
Filter Size |
Qty |
| FF01-378/52-32 |
Single Band Exciter |
32 x 5.0 mm |
1 |
| FF02-447/60-32 |
Single Band Emitter |
32 x 3.5 mm |
1 |
| FF409-Di03-32×44-FX |
Single Band Dichroic |
32 x 44 x 1.1 mm |
1 |
